Chronic stress modulates regional cerebral glucose transporter expression in an age-specific and sexually-dimorphic manner

• Authors: Sean Kelly, Emory University; Constance S. Harrell, Emory University; Gretchen Neigh, Emory University
• Journal Title: Physiology and Behavior
• Volume: Volume 126
• Publisher: Elsevier: 12 months | 2014-03-14, Pages 39-49
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1016/j.physbeh.2013.12.002
• Subjects: Psychology, Psychobiology; Biology, Physiology
• Keywords: Social Sciences; Science & Technology; Life Sciences & Biomedicine; Psychology, Biological; Behavioral Sciences; Psychology; Stress; Age; Sex; Brain; Glucose transporter; CENTRAL-NERVOUS-SYSTEM; BLOOD-BRAIN-BARRIER; HIPPOCAMPAL DENDRITIC SPINES; IMMATURE RAT-BRAIN; ADOLESCENT STRESS; POSTNATAL-DEVELOPMENT; ENERGY HOMEOSTASIS; HYPOXIA-ISCHEMIA; SEX-DIFFERENCES; MESSENGER-RNA

Abstract

Facilitative glucose transporters (GLUT) mediate glucose uptake across the blood-brain-barrier into neurons and glia. Deficits in specific cerebral GLUT isoforms are linked to developmental and neurological dysfunction, but less is known about the range of variation in cerebral GLUT expression in normal conditions and the effects of environmental influences on cerebral GLUT expression. Knowing that puberty is a time of increased cerebral plasticity, metabolic demand, and shifts in hormonal balance for males and females, we first assessed gene expression of five GLUT subtypes in four brain regions in male and female adolescent and adult Wistar rats. The data indicated that sex differences in GLUT expression were most profound in the hypothalamus, and the transition from adolescence to adulthood had the most profound effect on GLUT expression in the hippocampus. Next, given the substantial energetic demands during adolescence and prior demonstrations of the adverse effects of adolescent stress, we determined the extent to which chronic stress altered GLUT expression in males and females in both adolescence and adulthood. Chronic stress significantly altered cerebral GLUT expression in males and females throughout both developmental stages but in a sexually dimorphic and brain region-specific manner. Collectively, our data demonstrate that cerebral GLUTs are expressed differentially based on brain region, sex, age, and stress exposure. These results suggest that developmental and environmental factors influence GLUT expression in multiple brain regions. Given the importance of appropriate metabolic balance within the brain, further assessment of the functional implications of life stage and environmentally-induced changes in GLUTs are warranted.