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To whom correspondence should be addressed: Eric A. Ortlund, Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA. Telephone: (404) 727-5014. Fax: (404) 727-2738. Email: eric.ortlund@emory.edu.
P.M.M. purified, crystallized and determined both LRH-1 structures, measured phospholipid levels and carried out co-regulator-peptide interaction studies.
M.C.P. measured lipid phosphorus levels, optimized apo LRH-1 production and carried out co-regulator peptide interaction studies.
W.H.H. conducted reporter gene experiments.
J.L.L. and P.R.G. conducted thermal unfolding and HDX experiments.
P.M.M., J.L.L., P.R.G. and E.A.O. analyzed and interpreted the data.
P.M.M. and E.A.O. conceived the experiments and wrote the manuscript.
Paul M. Musille and Manish Pathak contributed equally.
We thank Dr. Nicholas T. Seyfried in the Dept. of Biochemistry at the Emory University for his help with acquiring the mass spectral data.
The authors declare no competing financial interests.
This work was supported with start up funds from Emory University.
PMM was supported by Emory-NIEHS Graduate and Postdoctoral Training in Toxicology (T32ES012870).
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