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Author Notes:

Corresponding author with complete address, including an email address: Dieter Jaeger Dept. Biology, 1510 Clifton Rd. Atlanta, GA 30322 Tel. 404 727 8139, djaeger@emory.edu

Contributor Information Thomas Sangrey, Dept. of Biology, Emory University, Atlanta, GA. Dieter Jaeger, Dept. of Biology, Emory University, Atlanta, GA.

Subject:

Research Funding:

This work was supported by the National Institutes of Health grants R01-MH065634 to DJ

Keywords:

  • rat
  • brain slice
  • dynamic clamp
  • inhibition
  • persistent ion channel

Journal Title:

European Journal of Neuroscience

Volume:

Volume 32, Number 10

Publisher:

, Pages 1646-1657

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Deep cerebellar nucleus (DCN) neurons show pronounced post-hyperpolarization rebound burst behavior, which may contribute significantly to responses to strong inhibitory inputs from cerebellar cortical Purkinje cells. Thus, rebound behavior could importantly shape the output from the cerebellum. We used whole cell recordings in brain slices to characterize DCN rebound properties and their dependence on hyperpolarization duration and depth. We found that DCN rebounds showed distinct fast and prolonged components, with different stimulus dependence and different underlying currents. The initial depolarization leading into rebound spiking was carried by HCN current, and variable expression of this current could lead to a control of rebound latency. The ensuing fast rebound burst was due to T-type calcium current, as previously described. It was highly variable between cells in strength, and could be expressed fully after short periods of hyperpolarization. In contrast, a subsequent prolonged rebound component required longer and deeper periods of hyperpolarization before it was fully established. We found using voltage-clamp and dynamic clamp analyses that a slowly inactivating persistent sodium current fit the conductance underlying this prolonged rebound component resulting in spike rate increases over several seconds. Overall, our results demonstrate that multiphasic DCN rebound properties could be elicited differentially by different levels of Purkinje cell activation, and thus create a rich repertoire of potential rebound dynamics in the cerebellar control of motor timing.

Copyright information:

© 2010 The Authors. European Journal of Neuroscience © 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd

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