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Author Notes:

To whom correspondence should be addressed (email dpjones@emory.edu).

ACKNOWLEDGMENTS: We thank Dr Yan Chen for assistance with Trx2 redox Western blot analysis. HaCaT cells were a gift from Bito Toshinori of the Kobe Graduate University School of Medicine, Kobe, Japan.

Subject:

Research Funding:

This work was supported by National Institutes of Health (NIH) grant ES011195. W.H.W. was supported in part by NIH grant ES012260. J.M.H. was supported in part by NIH grant ES013015.

W.H.W. was supported in part by NIH grant ES012260.

J.M.H. was supported in part by NIH grant ES013015.

Keywords:

  • epidermal growth factor (EGF)
  • glutathione
  • redox signalling
  • thioredoxin-1
  • thioredoxin-2
  • AMS, 4-acetoamido-4′-maleimidylstilbene-2,2′-disulphonic acid
  • ASK1, apoptosis signal-regulating kinase-1
  • DCFH-DA, dichlorofluorescein diacetate
  • DTT, dithiothreitol
  • Eh, redox potential
  • E′0, midpoint potential
  • EGF, epidermal growth factor
  • EGFR, EGF receptor
  • IAA, iodoacetic acid
  • Prx, peroxiredoxin
  • ROS, reactive oxygen species
  • TBS, Tris-buffered saline
  • Trx, thioredoxin

Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signalling

Tools:

Journal Title:

Biochemical Journal

Volume:

Volume 386, Number Pt 2

Publisher:

, Pages 215-219

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.

Copyright information:

© 2005 Biochemical Society

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