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Author Notes:

Address correspondence to: Elliot L. Chaikof, M.D., Ph.D., Department of Surgery, Beth Israel Deaconess Medical Center, 110 Francis St, Suite 9F, Boston, MA 02115, Tel: (617) 632-9581, echaikof@bidmc.harvard.edu

Subject:

Research Funding:

This work was supported by the NIH and the Juvenile Diabetes Research Foundation.

Use of Malvern Zetasizer was supported by the Wyss Institute of Biologically Inspired Engineering of Harvard.

Keywords:

  • Science & Technology
  • Technology
  • Engineering, Biomedical
  • Materials Science, Biomaterials
  • Engineering
  • Materials Science
  • Polypeptides
  • Protein micelles
  • Conjugation
  • Vascular permeability
  • Bioimaging
  • FLUORESCENT NANOPARTICLES
  • VASCULAR-PERMEABILITY
  • DRUG-DELIVERY
  • POLYMER
  • CANCER
  • BIOCOMPATIBILITY
  • NANOCARRIERS
  • ACCUMULATION
  • POLYPEPTIDES
  • ENDOTHELIUM

Amphiphilic protein micelles for targeted in vivo imaging

Tools:

Journal Title:

Acta Biomaterialia

Volume:

Volume 8, Number 7

Publisher:

, Pages 2476-2482

Type of Work:

Article | Post-print: After Peer Review

Abstract:

A variety of polymeric nanoparticles have been developed for bioimaging applications. This study reports on the use of a 50 nm recombinant protein nanoparticle with a multivalent surface as a vehicle for functionalization with a model imaging agent. Multiple fluorescent probes were covalently conjugated to surface amines of crosslinked amphiphilic elastin-mimetic protein micelles using N-hydroxysuccinimide ester chemistry. In vivo fluorescence imaging confirmed that protein micelles selectively accumulated at sites of angioplasty induced vessel wall injury, presumably via an enhanced permeability and retention effect. This investigation demonstrates the potential of amphiphilic protein micelles to be used as a vehicle for selective imaging of sites associated with a disrupted or leaky endothelium.

Copyright information:

© 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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