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Author Notes:
Correspondence: Dr. Pavel Bostik, Department of Pathology and Laboratory Medicine, Emory University, WMB rm#2337A, 101 Woodruff Circle, Atlanta, GA 30322; Email: pavel.bostik@emory.edu; Phone: 404-712-2835; Fax: 404-712-1771
Acknowledgments: We are grateful to Dr. Guido Silvestri for providing select PBMC samples and to Dr. Jorg Goronzy for his advice in establishing the assay.
Subjects:
Research Funding:
This work was supported by the NIH RO1 AI65362 to P.B.
Keywords:
- AIDS
- SIV
- T cell receptor
- V beta
- CD4+ T cell
Maintenance of CD4+ T cell TCR Vβ repertoire heterogeneity is characteristic of apathogenic SIV infection in non-human primate model of AIDS
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Abstract:
A PCR based assay was established to screen for potential changes in the heterogeneity of Vβ TCR expressing CD4+ T cells from rhesus macaques (RM) and sooty mangabeys (SM) prior to and following SIV infection. The rationale for these studies was to determine whether the progressive CD4+ T cell loss in SIV infected disease susceptible RM and the moderate CD4+ T cell loss in disease resistant SM leads to the depletion of select Vβ TCR families of CD4+ T cells. Results show that whereas SIV infection leads to the loss of Vβ TCR heterogeneity in disease susceptible RM, the CD4+ T cells from SM retain their degree of Vβ TCR heterogeneity, suggesting that the mechanism(s) of SIV induced CD4+ T cell loss maybe distinct in these 2 species and contribute to the differences in the clinical outcome.
Copyright information:
© 2007 Elsevier Inc. All rights reserved.
This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/3.0/).
