Epithelial Cell Specificity and Apotope Recognition by Serum Autoantibodies in Primary Biliary Cirrhosis

• Authors: Guanghua Rong, University of California; Renqian Zhong, Second Military Medical University; Ana Lleo, University of California; Patrick S.C. Leung, University of California; Christopher L. Bowlus, University of California; Guo-Xiang Yang, University of California; Chen-Yen Yang, University of California; Ross L. Coppel, Monash University; Aftab A Ansari, Emory University; Dean A. Cuebas, Missouri State University; Howard J. Worman, Columbia University; Pietro Invernizzi, University of California; Gregory J. Gores, The Mayo Clinic; Gary Norman, INOVA Diagnostics, Inc.; Xiao-Song He, University of California; M. Eric Gershwin, University of California
• Journal Title: Hepatology
• Volume: Volume 54, Number 1
• Publisher: Wiley-Blackwell | 2011-07, Pages 196-203
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1002/hep.24355
• Subjects: Health Sciences, Medicine and Surgery; Health Sciences, Pathology
• Keywords: Apoptosis; human intrahepatic biliary epithelial cells; E2 subunit of 2-oxoacid dehydrogenase complex; nuclear antigens; autoimmunity

Abstract

A major enigma of primary biliary cirrhosis (PBC) is the selective targeting of biliary cells. Our laboratory has reported that following apoptosis human intrahepatic biliary epithelial cells (HiBEC) translocate the E2 subunit of the pyruvate dehydrogenase complex immunologically intact into apoptotic bodies, forming an apotope. However, the cell type and specificity of this reaction has not been fully defined. To address this issue we have investigated whether PDC-E2, BCOADC-E2, OGDC-E2, four additional inner mitochondrial enzymes and four nuclear antigens remain immunologically intact with respect to post-apoptotic translocation in HiBEC and 3 additional control epithelial cells. We report that all three 2-oxo acid dehydrogenase enzymes share the ability to remain intact within the apotope of HiBEC. Interestingly the E2 subunit of the branched chain 2-oxo acid dehydrogenase complex also remained intact in the other cell types tested. We extended the data using 95 AMA+ and 19 AMA- PBC and 76 control sera for reactivity against the 7 mitochondrial proteins studied herein and also the ability of AMA- sera to react with HIBEC apotopes. Sera from 3/95 AMA+ sera, but none of the controls, reacted with 2, 4-dienoyl CoA reductase 1 (DECR1), an enzyme also present intact only in the HiBEC apotope; DECR1 has not been previously associated with any autoimmune disease. Finally the specificity of HIBEC apotope reactivity was confined to AMA+ sera. In conclusion, we submit that the biliary specificity of PBC is secondary to the unique processes of biliary apoptosis.