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Author Notes:

Correspondence and requests for materials should be addressed to J.M. (jmarco@cabm.rutgers.edu) and A.G (arash.grakoui@emory.edu).

See publication for full list of author contributions.

We acknowledge access to the X25 beamline at NSLS and thank the NSLS staff.

We thank J. Tainer and J. Perry for their support and access to SIBYLS beamline at the Advanced Light Source, Lawrence Berkeley National Laboratory.

We thank E. Arnold, J. Bonanno S.K. Burley, J. Chiu, D. Comoletti, E. Elrod, F. Jiang, S. Khare, A. Shatkin, A. Stock, J. Shires, and A. Thanou for providing helpful comments and assistance.

Special thanks to C.M. Rice for providing J6 HCV clone and support.

The authors declare no competing financial interests.


Research Funding:

NSLS is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC02-98CH10886.

This work was supported by a Yerkes Research Center Base Grant RR-00165 (A.G) and NIH grants P50 GM103368 (J.M.), R01 AI080659 (J.M.) PHS AI070101 (A.G), and DK083356 (A.G).

A.G.K. was supported by a grant from the New Jersey Commission On Cancer Research (DFHS13CRP001).


  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • E2
  • CELL

Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2

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Journal Title:



Volume 509, Number 7500


, Pages 381-+

Type of Work:

Article | Post-print: After Peer Review


Hepatitis C virus (HCV) is a significant public health concern with approximately 160 million people infected worldwide. HCV infection often results in chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. No vaccine is available and current therapies are effective against some, but not all, genotypes. HCV is an enveloped virus with two surface glycoproteins (E1 and E2). E2 binds to the host cell through interactions with scavenger receptor class B type I (SR-BI) and CD81, and serves as a target for neutralizing antibodies. Little is known about the molecular mechanism that mediates cell entry and membrane fusion, although E2 is predicted to be a class II viral fusion protein. Here we describe the structure of the E2 core domain in complex with an antigen-binding fragment (Fab) at 2.4Å resolution. The E2 core has a compact, globular domain structure, consisting mostly of β 2-strands and random coil with two small α-helices. The strands are arranged in two, perpendicular sheets (A and B), which are held together by an extensive hydrophobic core and disulphide bonds. Sheet A has an IgG-like fold that is commonly found in viral and cellular proteins, whereas sheet B represents a novel fold. Solution-based studies demonstrate that the full-length E2 ectodomain has a similar globular architecture and does not undergo significant conformational or oligomeric rearrangements on exposure to low pH. Thus, the IgG-like fold is the only feature that E2 shares with class II membrane fusion proteins. These results provide unprecedented insights into HCV entry and will assist in developing an HCV vaccine and new inhibitors.

Copyright information:

© 2014 Macmillan Publishers Limited.

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