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Author Notes:

Correspondence to: Dr. Alan J. Sokoloff, Department of Physiology, Emory University, 615 Michael Street, Atlanta, GA 30322, Asokolo@emory.edu.

The authors would like to thank Ms. Nirjari Dalal for technical assistance and Ms Sona Santos (California National Primate Research Center) for help with primate tissue acquisition.

Subject:

Research Funding:

This work was supported by grant DC005017 from the National Institute on Deafness and Other Communication Disorders to Dr. Alan J. Sokoloff.

Human tissue was kindly provided by the Emory University School of Medicine Body Donor Program or purchased from the National Disease Research Interchange.

Non-human primate tissue was purchased from the California National Primate Research Center (Research Center Base Grant RR00169, National Center for Research Resources, NIH).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Clinical Neurology
  • Neurosciences
  • Neurosciences & Neurology
  • human
  • myosin heavy chain
  • muscle
  • suprahyoid
  • swallowing
  • HUMAN LARYNGEAL MUSCLES
  • HUMAN DIGASTRIC MUSCLE
  • HUMAN MYLOHYOID MUSCLE
  • SKELETAL-MUSCLE
  • MASTICATORY MUSCLES
  • EXTRAOCULAR-MUSCLE
  • SLOW MYOSIN
  • FIBER-TYPE
  • ELECTROPHORETIC SEPARATION
  • DIFFERENTIAL EXPRESSION

Journal Title:

Muscle and Nerve

Volume:

Volume 49, Number 4

Publisher:

, Pages 534-544

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Introduction: Contradictory reports of the myosin heavy chain (MHC) composition of adult human suprahyoid muscles leave unresolved the extent to which these muscles express developmental and unconventional MHC. Methods: By immunohistochemistry, separation sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-Coomassie, separation SDS-PAGE-Western blot, and mRNA PCR, we tested for conventional MHCI, MHCIIA, MHCIIX, developmental MHC embryonic and MHC neonatal, and unconventional MHC alpha-cardiac, MHC extraocular, and MHC slow tonic in adult human anterior digastric (AD), geniohyoid (GH), and mylohyoid (MH) muscles. Results: By separation SDS-PAGE-Coomassie and Western blot, only conventional MHC are present. By immunohistochemistry all muscle fibers are positive for MHCI, MHCIIA, or MHCIIX, and fewer than 4 fibers/mm2 are positive for developmental or unconventional MHC. By PCR, mRNA of MHCI and MHCIIA dominate, with sporadically detectable MHC alpha-cardiac and without detectable mRNA of other developmental and unconventional MHC. Conclusions: We conclude that human suprahyoid muscles AD, GH, and MH are composed almost exclusively of conventional MHC isoforms.

Copyright information:

© 2013 Wiley Periodicals, Inc.

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