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Author Notes:

David Weinshenker, Ph.D. Department of Human Genetics, Emory University School of Medicine, 615 Michael St., Whitehead 301, Atlanta, GA 30322. Phone: (404) 727-3106, Fax: (404) 727-3949, dweinshenker@genetics.emory.edu

The authors declare no conflicts of interest.

Subjects:

Research Funding:

This work was supported by the National Institute of Drug Abuse (DA027535 to DW); the National Institute of General Medical Sciences (5K12GM000680 to DAM); and the NCRR base grant of the Yerkes National Primate Research Center (RR00165).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Neurosciences
  • Neurosciences & Neurology
  • alpha 1-adrenergic receptor
  • D1 dopamine receptor
  • prefrontal cortex
  • electron microscopy
  • catecholamine
  • VENTRAL TEGMENTAL AREA
  • D-5 DOPAMINE-RECEPTOR
  • SUBCELLULAR-LOCALIZATION
  • CELLULAR-LOCALIZATION
  • NUCLEUS-ACCUMBENS
  • ADENYLYL-CYCLASE
  • COCAINE-SEEKING
  • PRIMATE BRAIN
  • D-AMPHETAMINE
  • NEURONS

D1-DOPAMINE AND alpha 1-ADRENERGIC RECEPTORS CO-LOCALIZE IN DENDRITES OF THE RAT PREFRONTAL CORTEX

Tools:

Journal Title:

Neuroscience

Volume:

Volume 258

Publisher:

, Pages 90-100

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Functional interactions between dopaminergic and noradrenergic systems occur in many brain areas, including the prefrontal cortex (PFC). Biochemical, electrophysiological and behavioral data indicate crosstalk between D1 dopamine receptor (D1R) and α1-adrenergic receptor (α1AR) signaling in the PFC. However, it is unknown whether these interactions occur within the same neurons, or between neurons expressing either receptor. In this study, we used electron microscopy immunocytochemistry to demonstrate that D1Rs and α1ARs co-localize in rat PFC neuronal elements, most prominently in dendrites (60-70%), but also significantly in axon terminals, unmyelinated axons and spines (~20-30%). Our data also showed that the ratio of plasma membrane-bound to intracellular α1ARs is significantly reduced in D1R-expressing dendrites. Similar results were obtained using either a pan-α1AR or a selective α1bAR antibody to label noradrenergic receptors. Thus, these results demonstrate that D1Rs and α1ARs co-localize in PFC dendrites, thereby suggesting that the catecholaminergic effects on PFC function may be driven, at least in part, by cell-autonomous D1R-α1AR interactions.

Copyright information:

© 2013 IBRO.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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