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Author Notes:

Correspondence: Ning Xiao, Institute of Parasitic Diseases, Sichuan Center for Disease Control and Prevention, Chengdu, Sichuan 610041, PR China; Email: ningxiao116@yahoo.com.cn.

Author Contributions: NX, JR, PB and DB conceived of and designed the study, and were assisted by DQ and RS who coordinated the study.

NX and YL carried out the molecular genetic studies, and were assisted by JR, PB and DB in analysing the results.

NX, JR, PB and DB drafted the manuscript.

All authors read and approved the final manuscript.

Acknowledgments: The authors wish to thank Kang Junxing, Director of the Sichuan Center for Disease Control and Prevention (Chengdu, People's Republic of China) for his continued support and collaboration.

The authors also wish to acknowledge Zhong Bo, Ye Hong, Cui Lina, Chen Lin, Zhang Yi, Meng Xianhong of the Sichuan Center for Disease Control and Prevention for their contribution to the laboratory work.

Disclosures: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

The authors declare that they have no competing interests.

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Research Funding:

This work was supported in part by the NIH/NSF Ecology of Infectious Disease Program (grant no. 0622743) and the Emory Global Health Institute.

Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource

Tools:

Journal Title:

Parasites and Vectors

Volume:

Volume 4, Number 13

Publisher:

, Pages 1-4

Type of Work:

Article | Final Publisher PDF

Abstract:

Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of Schistosoma populations. We identified many microsatellite loci in the draft genome of Schistosoma japonicum using defined search criteria and selected a subset for further analysis. From an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worms from endemic areas. All but one of the selected microsatellites contain simple tri-nucleotide repeats. Moderate to high levels of polymorphism were detected. Numbers of alleles ranged from 6 to 14 and observed heterozygosity was always >0.6. The loci reported here will facilitate high resolution population-genetic studies on schistosomes in re-emergent foci.

Copyright information:

© 2011 Xiao et al; licensee BioMed Central Ltd.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 2.0 Generic License (http://creativecommons.org/licenses/by/2.0/).

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