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Author Notes:

Correspondence: Gary J Bassell: gary.bassell@emory.edu

Authors' Contributions: SAS contributed to the conception and design of the study, tested and validated the method, performed and analyzed the live imaging experiments, performed the statistical analyses, and drafted the manuscript.

XY performed the mouse neuron culture, transfection, and imaging for the LY294002 experiments.

CG participated in the design and analysis of the LY294002 experiments.

GJB contributed to the conception, analysis, and coordination of the study, and edited the manuscript.

All authors have read and approved the final manuscript.

Acknowledgments: The authors would like to thank Dr. Alexa Mattheyses for technical assistance and critically reading the manuscript and Andrew Swanson for technical assistance and helpful discussions.

Disclosures: CG and GJB declare that they are inventors on patent application PCT/US2010/055387.

Subjects:

Research Funding:

SAS was supported by predoctoral fellowships from the NIH (F31NS063668, T32GM0860512 and T32NS007480) and the Epilepsy Foundation and Lennox & Lombroso Trust Fund.

CG was supported by a Basic Science Grant from NFXF.

This work was supported by NIH MH085617 and NARSAD Investigator Award to GJB, the Emory/Baylor Fragile X Center (P30HD024064) and the Neuronal Imaging Core of the Emory Neuroscience NINDS Core Facility (P30NS055077).

Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model

Journal Title:

Molecular Brain

Volume:

Volume 4, Number 38

Publisher:

, Pages 1-14

Type of Work:

Article | Final Publisher PDF

Abstract:

Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities in a disease model. We anticipate that this approach will advance studies of synapse structure and function in brain development, plasticity, and disease.

Copyright information:

© 2011 Swanger et al; licensee BioMed Central Ltd.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 2.0 Generic License (http://creativecommons.org/licenses/by/2.0/).

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