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Author Notes:

Requests for reprints: Hyunsuk Shim, Winship Cancer Institute, 1701 Uppergate Drive, C5008, Atlanta, GA 30322. Phone: 404-778-4564; Fax: 404-778-5550; hyunsuk_shim@emory.org.

We thank Drs. Jay Umbreit, Georgia Chen, and Daniel Brat at Emory University for critical reading of the manuscript.

Subjects:

Research Funding:

Grant support: Georgia Cancer Coalition Distinguished Cancer Scientist Development Fund (H. Shim) and the AACR-Cancer Research and Prevention Foundation Fellowship in Cancer Prevention Research (Z. Liang).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Oncology
  • SMALL INTERFERING RNAS
  • MAMMALIAN-CELLS
  • INHIBITION
  • MICE
  • SUPPRESSION
  • EXPRESSION
  • DELIVERY
  • SIRNAS
  • POTENT

Silencing of CXCR4 blocks breast cancer metastasis

Tools:

Journal Title:

Cancer Research

Volume:

Volume 65, Number 3

Publisher:

, Pages 967-971

Type of Work:

Article | Post-print: After Peer Review

Abstract:

RNA interference technology, silencing targeted genes in mammalian cells, has become a powerful tool for studying gene function. For the first time in cancer research, we show that direct injection of a pool of naked small interfering RNA (siRNA) duplexes can prevent tumorigenesis in an animal model, suggesting a novel preventive and therapeutic strategy for cancer management. As a model system, we used siRNA duplexes of CXCR4 to block breast cancer metastasis. Here, we show that blocking CXCR4 expression at the mRNA level by a combination of two siRNAs impairs invasion of breast cancer cells in Matrigel invasion assay and inhibits breast cancer metastasis in an animal model. Targeting more than one site of the target gene may be important to overcome the functional redundancy of other variants of a single gene, especially in in vivo experiments. Moreover, our studies confirm the necessity of CXCR4 in breast cancer metastasis.

Copyright information:

©2005 American Association for Cancer Research.

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