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Author Notes:

Corresponding author. Email: bkick86@gmail.com

We thank Maya Encantada Meeks, Kristy Calderon, Michelle Hull, and Amy Dryman for their technical assistance.

Thank you to Dr Susan Sanchez and the Athens Veterinary Diagnostic Laboratory at the University of Georgia– Athens.

Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Veterinary Sciences
  • Zoology
  • SURGICAL SITE INFECTIONS
  • CHLORHEXIDINE GLUCONATE
  • POVIDONE-IODINE
  • BACTERIAL-FLORA
  • SURGERY
  • GUIDELINES
  • ANTISEPSIS
  • ALCOHOL
  • RATES
  • CATS

Evaluation of 4 Presurgical Skin Preparation Methods in Mice

Tools:

Journal Title:

Journal of the American Association for Laboratory Animal Science : JAALAS

Volume:

Volume 58, Number 1

Publisher:

, Pages 71-77

Type of Work:

Article | Final Publisher PDF

Abstract:

Mice routinely undergo surgical procedures for use in research; however, studies of skin preparation methods to achieve antisepsis are rare. The present study evaluated 4 skin preparation treatments: depilatory agent followed by povidone-iodine and alcohol scrub; depilatory agent followed by povidone-iodine and saline scrub; electric clippers followed by povidone-iodine and alcohol scrub; and electric clippers followed by povidone-iodine and saline scrub. Swabs for bacterial culture were obtained immediately after hair removal and after scrubbing to measure the reduction in bacterial load. Full-thickness incisions were assigned ASEPSIS wound scores and examined histologically on days 0, 1, and 7 after surgery. Neither bacterial load growth nor ASEPSIS wound scores differed among any of the treatments. Histopathology revealed statistically significant but biologically irrelevant differences. Overall all treatment methods achieved acceptable bacterial load reduction and surgical site healing.
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