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Author Notes:

Charles Stebbins, PhD, Department of Internal Medicine, Division of Cardiovascular Medicine, One Shields Ave, TB-172, University of California, Davis, Davis, CA 95616, Phone: 530-752-4714, Fax: 530-752-3264, clstebbins@ucdavis.edu

Subjects:

Research Funding:

This work was funded by a Pilot and Feasibility Grant from the UC-Davis Clinical Nutrition Research Unit (NIDDK 35747, Dr. Charles H. Halsted, P.I.); and NIH National Institute of Aging Grant AG19327 (AAK).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cardiac & Cardiovascular Systems
  • Pharmacology & Pharmacy
  • Cardiovascular System & Cardiology
  • phospho-eNOS
  • Akt kinase
  • phospho-Akt
  • HSP90
  • 3-nitrotyrosine
  • cell culture
  • NITRIC-OXIDE SYNTHASE
  • CHRONIC HEART-FAILURE
  • POLYUNSATURATED FATTY-ACIDS
  • FISH-OIL-RICH
  • DOCOSAHEXAENOIC ACID
  • MOLECULAR-MECHANISMS
  • SHOCK PROTEINS
  • SHEAR-STRESS
  • IN-VIVO
  • ACTIVATION

Effects of Dietary Decosahexaenoic Acid (DHA) on eNOS in Human Coronary Artery Endothelial Cells

Tools:

Journal Title:

Journal of Cardiovascular Pharmacology and Therapeutics

Volume:

Volume 13, Number 4

Publisher:

, Pages 261-268

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Endothelial dysfunction occurs in heart disease and may reduce functional capacity via attenuations in peripheral blood flow. Dietary decosahexaenoic acid (DHA) may improve this dysfunction, but the mechanism is unknown. This study determined if DHA enhances expression and activity of eNOS in cultured human coronary artery endothelial cells (HCAEC). HCAEC from 4 donors were treated with 5 nM, 50 nM, or 1 μM DHA for 7 days to model chronic DHA exposure. A trend for increased expression of endothelial nitric oxide synthase (eNOS) and phospho-eNOS was observed with 5 and 50 nM DHA. DHA also enhanced expression of 2 proteins instrumental in activation of eNOS: phospho-Akt (5 and 50 nM) and HSP90 (50 nM and 1 μM). Vascular endothelial growth factor-induced activation of Akt increased NOx in treated (50 nM DHA) versus untreated HCAEC (9.2 ± 1.0 vs 3.3 ± 1.1 μmol/μg protein/μL). Findings suggest that DHA enhances eNOS and Akt activity, augments HSP90 expression, and increases NO bioavailability in response to Akt kinase activation.

Copyright information:

© 2008 Sage Publications.

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