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Author Notes:

Anthony W.S. Chan, Yerkes National Primate Research Center, Rm. 2212 Neuroscience Research Bldg., 954 Gatewood Rd. N.E., Atlanta, GA 30329, USA. achan@genetics.emory.edu.

The authors thank Dr. Carlos Lois for providing lentiviral vector backbone; Jin-Jing Yang and Tina Huang for their technical assistance.

The authors also thank Katherine Larkin and Leslee Sinclair for the proofreading; Fang-Chueh Liu and Chanchao Lorthongpanich for statistic analysis; and Grey Tharp for computer support.

Subjects:

Research Funding:

Contract grant sponsor: NIH (Yerkes National Primate Research Center), Contract grant number: RR-00165. Contract grant sponsor: NCRR/NIH, Contract grant number: RR018827-04.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Developmental Biology
  • Genetics & Heredity
  • lentiviral vector
  • transgenesis
  • integration
  • preference genome
  • mouse zygote
  • IMMUNODEFICIENCY-VIRUS TYPE-1
  • OVINE PULMONARY ADENOCARCINOMA
  • PSEUDOTYPED RETROVIRAL VECTORS
  • GENE-TRANSFER
  • HUMAN GENOME
  • CHROMOSOMAL POSITION
  • SITE SELECTION
  • HIGH-TITER
  • GERM LINE
  • IN-VITRO

Lentiviral Integration Preferences in Transgenic Mice

Tools:

Journal Title:

genesis

Volume:

Volume 46, Number 12

Publisher:

, Pages 711-718

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Lentiviral gene transfer has a significant impact on the development of biomedical research. One of the most important features of lentiviruses is the capability to infect both dividing and nondividing cells. However, little is known whether integration preference exists, specifically in early embryos. An in-depth genome analysis on 112 independent lentiviral integration sites from 43 transgenic founder mice was performed to determine if there are preferable sites for lentiviral integration in early embryonic genome. Our results demonstrated that lentiviruses were biased in integrating within intragenic regions, especially in the introns. However, no integration preference was found associated with specific chromosomes, repetitive elements, or CpG islands, nor was there any preference for integrating at close proximity to transcription start sites. Our findings suggested that lentiviruses were biased to integrate into the intragenic regions of early embryonic genome of mouse.

Copyright information:

© 2008 Wiley-Liss, Inc.

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