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Author Notes:

Corresponding author. Mailing address: Gladstone Institute of Virology and Immunology, 1650 Owens Street, San Francisco, CA 94158. Phone: (415) 734-4824. Fax: (415) 355-0153. mcavrois@gladstone.ucsf.edu

We thank Mario Santiago and Kara Lassen for discussions, Stephen Ordway and Gary Howard for editorial assistance, John C.W. Carroll for graphic arts, and Sue Cammack and Robin Givens for administrative assistance.

We also thank the NIH AIDS Research & Reference Reagent Program, Division of AIDS, NIAID, NIH for the vectors expressing various primary envelopes.

Subjects:

Research Funding:

These studies were supported by funding from the National Institutes of Health (P01 AI083050).

The authors also acknowledge the UCSF-GIVI CFAR for infrastructure support (P30 AI27763).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemical Research Methods
  • Biochemistry & Molecular Biology
  • HIV fusion
  • Primary envelopes
  • CD4 T-cells
  • Monocyte-derived dendritic cells
  • VIRUS TYPE-1 ENVELOPE
  • NECROSIS-FACTOR-ALPHA
  • ENDOPLASMIC-RETICULUM
  • ENDOPROTEOLYTIC CLEAVAGE
  • INTRACELLULAR-TRANSPORT
  • DENDRITIC CELLS
  • GLYCOPROTEIN
  • NEUTRALIZATION
  • INFECTIVITY
  • SENSITIVITY

Measuring HIV fusion mediated by envelopes from primary viral isolates

Tools:

Journal Title:

Methods

Volume:

Volume 53, Number 1

Publisher:

, Pages 34-38

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Over the course of infection, the human immunodeficiency virus type 1 (HIV-1) continuously adapts in part to evade the host's neutralizing antibody response. Antibodies often target the HIV envelope proteins that mediate HIV fusion to its cellular targets. HIV virions pseudotyped with primary envelopes have often been used to explore the fusogenic properties of these envelopes. Unfortunately, these pseudotyped virions fuse with greatly reduced efficiency to primary cells. Here, we describe a relatively simple strategy to clone primary envelopes into a provirus and increase the sensitivity of the virion-based fusion assay.

Copyright information:

© 2010 Elsevier Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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