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Author Notes:

Correspondence: Email: prida@gsu.edu Email: raneja@gsu.edu

Author Contributions: R.A. and P.C.G.R. conceived the study and designed the experiments. R.A. supervised the project.

A.O., R.O., and S.K. performed statistical analyses. N.W., K.M., S.B., and A.R. processed, stained, and handled clinical specimens.

X.L., G.O., and M.G. scored the clinical specimens. C.S.W., M.J., and S.P. collected and managed clinical data.

C.G. and R.C.T. performed mechanistic experiments.

A.O. and P.C.G.R. wrote the manuscript. U.K., M.D.R., A.R., G.C., and R.A. critically revised the manuscript.

Acknowledgments: We gratefully acknowledge Dr. Yuan Liu, (Emory University) for assistance with initial statistical analyses, and Dr. Claire Walczak (Indiana University) for generously providing the KIFC1 antibody.

Subjects:

Research Funding:

This work was supported by grants to RA by from the NCI (R01 CA169127) and NIMHD (R41 MD010303).

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • VESICLE TRANSPORT
  • EXTRA CENTROSOMES
  • KINESIN
  • SPINDLE
  • CELLS
  • HSET
  • PROTEIN
  • MICROTUBULES
  • MECHANISMS
  • ETHNICITY

Multi-institutional study of nuclear KIFC1 as a biomarker of poor prognosis in African American women with triple-negative breast cancer

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Journal Title:

Scientific Reports

Volume:

Volume 7, Number 1

Publisher:

, Pages 42289-42289

Type of Work:

Article | Final Publisher PDF

Abstract:

Nuclear KIFC1 (nKIFC1) predicts worse outcomes in breast cancer, but its prognostic value within racially distinct triple-negative breast cancer (TNBC) patients is unknown. Thus, nKIFC1 expression was assessed by immunohistochemistry in 163 African American (AA) and 144 White TNBC tissue microarrays (TMAs) pooled from four hospitals. nKIFC1 correlated significantly with Ki67 in White TNBCs but not in AA TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. High nKIFC1 weighted index (WI) was associated with significantly worse overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS) (Hazard Ratios [HRs] = 3.5, 3.1, and 3.8, respectively; P = 0.01, 0.009, and 0.007, respectively) in multivariable Cox models in AA TNBCs but not White TNBCs. Furthermore, KIFC1 knockdown more severely impaired migration in AA TNBC cells than White TNBC cells. Collectively, these data suggest that nKIFC1 WI an independent biomarker of poor prognosis in AA TNBC patients, potentially due to the necessity of KIFC1 for migration in AA TNBC cells.

Copyright information:

© 2017 The Author(s).

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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