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Author Notes:

Corresponding author: Dr. Irma Van Die, Department of Molecular Cell Biology & Immunology, VU University Center, Van der Boechorstraat 7, 1081BT, Amsterdam, The Netherlands, Phone: +31204448157; im.vandie@vumc.nl.

We are indebted with Dr. Y. Guruz for providing sera from the outbreak in Turkey and Dr. J. Van Hellemond from the Rotterdam Harbour Hospital/Erasmus, the Netherlands for providing sera from patients with schistosomiasis and strongyloidiasis.

Subjects:

Research Funding:

We thank Core H of the Consortium of Functional Glycomics (Emory University School of Medicine, Atlanta, USA) and the support of the NIH/NHLBI Grant (GM) GM62116 for assistance in performing these studies.

Part of the work was supported by funding from the Dutch Technology Foundation (STW) to IvD and BT.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Parasitology
  • Serology
  • Glycosylation
  • Helminth
  • Glycan microarray
  • Trichinella spiralis
  • CIRCULATING CATHODIC ANTIGEN
  • SURFACE-PLASMON RESONANCE
  • TRICHINELLA-SPIRALIS
  • SCHISTOSOMA-MANSONI
  • CAENORHABDITIS-ELEGANS
  • CARBOHYDRATE EPITOPES
  • ANTIBODY-RESPONSES
  • MOLECULAR-CLONING
  • N-GLYCANS
  • DIAGNOSIS

Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis

Tools:

Journal Title:

Experimental Parasitology

Volume:

Volume 129, Number 3

Publisher:

, Pages 221-226

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods; however, few synthetic antigens with sufficient sensitivity and specificity are available. Here we evaluated a glycan microarray approach to select for synthetic glycan antigens that could be used for serodiagnosis of parasitic infections. Using a glycan array containing over 250 different glycan antigens, we identified GalNAcβ1-4(Fucα1-3)GlcNAc-R (LDNF) as a glycan antigen that is recognized by antibodies from Trichinella-infected individuals. We synthesized a neoglycoconjugate, consisting of five LDNF molecules covalently coupled to bovine serum albumin (BSA), and used this neoglycoconjugate as an antigen to develop a highly sensitive total-Ig ELISA for serological screening of trichinellosis. The results indicate that glycan microarrays constitute a promising technology for fast and specific identification of parasite glycan antigens to improve serodiagnosis of different parasitic infections, either using an ELISA format, or parasite-specific glycan arrays.

Copyright information:

© 2011 Elsevier Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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