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Author Notes:

E.L. Chaikof, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, 110 Francis Street, Suite 9F, Boston, MA 02215, USA. Tel.: +1 (617) 632 9581. echaikof@bidmc.harvard.edu

Subjects:

Keywords:

  • Amino Acid Sequence
  • Animals
  • Biocompatible Materials
  • Cell Adhesion
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Elastin
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Maleimides
  • Mesenchymal Stem Cells
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Oligopeptides
  • Peptides
  • Sulfhydryl Compounds
  • Sus scrofa

Maleimide-thiol coupling of a bioactive peptide to an elastin-like protein polymer

Tools:

Journal Title:

Acta Biomaterialia

Volume:

Volume 8, Number 2

Publisher:

, Pages 627-635

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Recombinant elastin-like protein (ELP) polymers display several favorable characteristics for tissue repair and replacement as well as drug delivery applications. However, these materials are derived from peptide sequences that do not lend themselves to cell adhesion, migration, or proliferation. This report describes the chemoselective ligation of peptide linkers bearing the bioactive RGD sequence to the surface of ELP hydrogels. Initially, cystamine is conjugated to ELP, followed by the temperature-driven formation of elastomeric ELP hydrogels. Cystamine reduction produces reactive thiols that are coupled to the RGD peptide linker via a terminal maleimide group. Investigations into the behavior of endothelial cells and mesenchymal stem cells on the RGD-modified ELP hydrogel surface reveal significantly enhanced attachment, spreading, migration and proliferation. Attached endothelial cells display a quiescent phenotype.

Copyright information:

© 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Creative Commons License

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