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Author Notes:

Correspondence: Zhigao Bu, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China; Email: zgbu@yahoo.com.

Authors' Contributions: LT, HZ and TH carried out the plasmid construct cloning, rescued and characterized the recombinant virus.

LT, JG, XW and ZW carried out the immunization assay and data analysis.

BZ and DK participated in the animal immunization.

ZB designed the whole study, provided general supervision and prepared the manuscript.

CY participated in experiment design and helped preparing the manuscript.

All authors have read and approved the submitted manuscript.

Acknowledgments: We thank Susan Watson for editing the manuscript.

Disclosures: The authors declare that they have no competing interests.


Research Funding:

This work was supported by Chinese National S&T Plan (2009ZX10004-214), by the grant from Chinese Ministry of Agriculture (200803014) and by the GHI program of Emory University.


  • Rabies virus
  • LEP
  • recombinant
  • inactivated vaccine

Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production


Journal Title:

Virology Journal


Volume 8, Number 454


, Pages 1-7

Type of Work:

Article | Final Publisher PDF


The rabies Flury Low Egg Passage virus (LEP) has been widely used as a seed virus to generate inactive vaccine. Here, we established a reverse genetic system for LEP and generated a recombinant LEP virus (rLEP-G) that carries two identical G genes. This recombinant virus showed similar properties to those of LEP with respect to in vitro growth, neurotropism index, and virulence in mice. rLEP-G produced 4.3-fold more G protein than did LEP in BHK-21 cells. The inactivated vaccine generated from rLEP-G induced significantly higher virus neutralization titers in mice and dogs than those produced in response to LEP-derived vaccine. Our results suggest that rLEP-G is an improved seed virus candidate for inactivated rabies virus vaccine manufacture.

Copyright information:

© 2011 Tao et al; licensee BioMed Central Ltd.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 2.0 Generic License (http://creativecommons.org/licenses/by/2.0/).

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