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Author Notes:

Correspondence: Indrajit Chowdhury, Department of Obstetrics and Gynecology, Morehouse School of Medicine, Atlanta, Georgia 30310. Email: indrajitfbs@gmail.com

I. C. and S. B. contributed to study concept and design, acquisition, analysis and interpretation of data, statistical analysis, and drafting of the manuscript.

W. Z. and S. M. contributed experimental support.

C. N. and N. S. contributed patient samples.

W. E. T., R. N. T., N. S., and A. D. contributed analysis and interpretation of data and critical revision of the manuscript for important intellectual content.

The authors declare that there are no conflicts of interest.

Subjects:

Research Funding:

This study was supported in part by National Institutes of Health Grants 1SC3 GM113751, U01 HD66439, 1R01HD057235, U54 CA118948, HD41749, S21MD000101, and G12‐RR03034.

This investigation was conducted in a facility constructed with support from Research Facilities Improvement Grant #C06 RR18386 from NIH/NCRR.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cell Biology
  • Physiology
  • curcumin
  • endometriosis
  • human
  • stromal cell
  • PERITONEAL-FLUID CONCENTRATIONS
  • QUALITY-OF-LIFE
  • AUTOCRINE MANNER
  • GENE-EXPRESSION
  • CHEMOKINES
  • WOMEN
  • PATHOGENESIS
  • APOPTOSIS
  • DIAGNOSIS
  • SURVIVAL

Curcumin attenuates proangiogenic and proinflammatory factors in human eutopic endometrial stromal cells through the NF-kappa B signaling pathway

Tools:

Journal Title:

Journal of Cellular Physiology

Volume:

Volume 234, Number 5

Publisher:

, Pages 6298-6312

Type of Work:

Article | Final Publisher PDF

Abstract:

Endometriosis is a chronic gynecological inflammatory disorder in which immune system dysregulation is thought to play a role in its initiation and progression. Due to altered sex steroid receptor concentrations and other signaling defects, eutopic endometriotic tissues have an attenuated response to progesterone. This progesterone-resistance contributes to lesion survival, proliferation, pain, and infertility. The current agency-approved hormonal therapies, including synthetic progestins, GnRH agonists, and danazol are often of limited efficacy and counterproductive to fertility and cause systemic side effects due to suppression of endogenous steroid hormone levels. In the current study, we examined the effects of curcumin (CUR, diferuloylmethane), which has long been used as an anti-inflammatory folk medicine in Asian countries for this condition. The basal levels of proinflammatory and proangiogenic chemokines and cytokines expression were higher in primary cultures of stromal cells derived from eutopic endometrium of endometriosis (EESC) subjects compared with normal endometrial stromal cells (NESC). The treatment of EESC and NESC with CUR significantly and dose-dependently reduced chemokine and cytokine secretion over the time course. Notably, CUR treatment significantly decreased phosphorylation of the IKKα/β, NF-κB, STAT3, and JNK signaling pathways under these experimental conditions. Taken together, our findings suggest that CUR has therapeutic potential to abrogate aberrant activation of chemokines and cytokines, and IKKα/β, NF-κB, STAT3, and JNK signaling pathways to reduce inflammation associated with endometriosis.

Copyright information:

© 2018 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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