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Author Notes:

C. Ross Either:315 Ferst Drive, 2306 IBB Atlanta, GA 30332-0363, ross.ethier@bme.gatech.edu, Phone: (404) 385-0100,Fax: (404) 385-1397.

Subjects:

Research Funding:

We gratefully acknowledge funding support from the Georgia Research Alliance.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Ophthalmology
  • Trabecular meshwork
  • Stiffness
  • Glaucoma
  • Outflow facility
  • Biomechanics
  • CONVENTIONAL OUTFLOW FACILITY
  • AQUEOUS-HUMOR OUTFLOW
  • INTRAOCULAR-PRESSURE
  • MECHANOTRANSDUCERS YAP
  • EXTRACELLULAR-MATRIX
  • SUBSTRATUM STIFFNESS
  • INTRINSIC STIFFNESS
  • KINASE INHIBITOR
  • CILIARY MUSCLE
  • LATRUNCULIN-B

Trabecular meshwork stiffness in glaucoma

Tools:

Journal Title:

Experimental Eye Research

Volume:

Volume 158

Publisher:

, Pages 3-12

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Alterations in stiffness of the trabecular meshwork (TM) may play an important role in primary open-angle glaucoma (POAG), the second leading cause of blindness. Specifically, certain data suggest an association between elevated intraocular pressure (IOP) and increased TM stiffness; however, the underlying link between TM stiffness and IOP remains unclear and requires further study. We here first review the literature on TM stiffness measurements, encompassing various species and based on a number of measurement techniques, including direct approaches such as atomic force microscopy (AFM) and uniaxial tension tests, and indirect methods based on a beam deflection model. We also briefly review the effects of several factors that affect TM stiffness, including lysophospholipids, rho-kinase inhibitors, cytoskeletal disrupting agents, dexamethasone (DEX), transforming growth factor-β2 (TGF-β2), nitric oxide (NO) and cellular senescence. We then describe a method we have developed for determining TM stiffness measurement in mice using a cryosection/AFM-based approach, and present preliminary data on TM stiffness in C57BL/6J and CBA/J mouse strains. Finally, we investigate the relationship between TM stiffness and outflow facility between these two strains. The method we have developed shows promise for further direct measurements of mouse TM stiffness, which may be of value in understanding mechanistic relations between outflow facility and TM biomechanical properties.

Copyright information:

© 2016 Elsevier Ltd

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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