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Author Notes:

Donald J. Noble djnoble@emory.edu Shawn Hochman shochm2@emory.edu

DN and SH were responsible for experimental design/execution and wrote the majority of the finished manuscript.

WG designed the LabVIEW feedback program and contributed extensively to experimental methods.

SG and KM ran nociception assays and helped with data analysis and interpretation.

All authors participated in manuscript edits and approved the final version.

The authors would like to thank Gretchen Neigh's laboratory for performing corticosterone assays and Michael Sawchuk for helping to troubleshoot early difficulties with the plethysmography setup.

Conflict of interest statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Subject:

Research Funding:

This research was supported by the Emory University Scholars Program in Interdisciplinary Neuroscience Research.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Physiology
  • operant conditioning
  • slow
  • deep breathing
  • experimental stressors
  • animal model
  • physio-behavioral monitoring
  • ARTERIAL BAROREFLEX SENSITIVITY
  • HEART-RATE-VARIABILITY
  • CHRONIC MILD STRESS
  • MINDFULNESS MEDITATION
  • ESSENTIAL-HYPERTENSION
  • VENTILATORY RESPONSES
  • MELATONIN SECRETION
  • NERVOUS-SYSTEM
  • BLOOD-PRESSURE
  • RESPIRATION

Slow Breathing Can Be Operantly Conditioned in the Rat and May Reduce Sensitivity to Experimental Stressors

Tools:

Journal Title:

Frontiers in Physiology

Volume:

Volume 8, Number OCT

Publisher:

, Pages 854-854

Type of Work:

Article | Final Publisher PDF

Abstract:

In humans, exercises involving slowed respiratory rate (SRR) counter autonomic sympathetic bias and reduce responses to stressors, including in individuals with various degrees of autonomic dysfunction. In the rat, we examined whether operant conditioning could lead to reductions in respiratory rate (RR) and performed preliminary studies to assess whether conditioned SRR was sufficient to decrease physiological and behavioral responsiveness to stressors. RR was continuously monitored during 20 2-h sessions using whole body plethysmography. SRR conditioned, but not yoked control rats, were able to turn off aversive visual stimulation (intermittent bright light) by slowing their breathing below a preset target of 80 breaths/min. SRR conditioned rats greatly increased the incidence of breaths below the target RR over training, with average resting RR decreasing from 92 to 81 breaths/min. These effects were significant as a group and vs. yoked controls. Preliminary studies in a subset of conditioned rats revealed behavioral changes suggestive of reduced reactivity to stressful and nociceptive stimuli. In these same rats, intermittent sessions without visual reinforcement and a post-training priming stressor (acute restraint) demonstrated that conditioned rats retained reduced RR vs. controls in the absence of conditioning. In conclusion, we present the first successful attempt to operantly condition reduced RR in an animal model. Although further studies are needed to clarify the physio-behavioral concomitants of slowed breathing, the developed model may aid subsequent neurophysiological inquiries on the role of slow breathing in stress reduction.

Copyright information:

© 2017 Noble, Goolsby, Garraway, Martin and Hochman.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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