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Author Notes:

Corresponding author: The Emory Clinic, 1365 Clifton Road, Suite 6200, Atlanta, GA 30322, rgross@emory.edu, Phone: 404-727-2354.

J.K.T, F.H.S, R.E.G. designed experiments.

J.K.T, F.H.S, K.D. conducted experiments and analyzed data.

J.K.T, F.H.S, K.D., R.E.G. wrote the manuscript.

The authors would like to thank Drs. Claire-Anne Gutekunst, Ken Berglund, Shan Ping Yu, and Ling Wei for their helpful feedback and guidance.

Special thanks to the Emory viral vector core and the rest of the Gross lab for their technical support.

Competing interests: none

Subjects:

Research Funding:

NS079268 and NS079757 to R.E.G., NS086433 to J.K.T.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Neurosciences
  • Neurosciences & Neurology
  • Epilepsy
  • Optogenetics
  • Networks
  • Circuits
  • Luminopsin
  • DEEP BRAIN-STIMULATION
  • ELECTRICAL-STIMULATION
  • ANTERIOR NUCLEUS
  • NEURAL CIRCUITS
  • DENTATE GYRUS
  • THALAMUS
  • RAT
  • EPILEPSIES
  • CORTEX

Chemically activated luminopsins allow optogenetic inhibition of distributed nodes in an epileptic network for non-invasive and multi-site suppression of seizure activity

Tools:

Journal Title:

Neurobiology of Disease

Volume:

Volume 109, Number Pt A

Publisher:

, Pages 1-10

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Although optogenetic techniques have proven to be invaluable for manipulating and understanding complex neural dynamics over the past decade, they still face practical and translational challenges in targeting networks involving multiple, large, or difficult-to-illuminate areas of the brain. We utilized inhibitory luminopsins to simultaneously inhibit the dentate gyrus and anterior nucleus of the thalamus of the rat brain in a hardware-independent and cell-type specific manner. This approach was more effective at suppressing behavioral seizures than inhibition of the individual structures in a rat model of epilepsy. In addition to elucidating mechanisms of seizure suppression never directly demonstrated before, this work also illustrates how precise multi-focal control of pathological circuits can be advantageous for the treatment and understanding of disorders involving broad neural circuits such as epilepsy.

Copyright information:

© 2017 Elsevier Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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