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Author Notes:

Correspondence Yoskaly Lazo‐Fernandez, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, The University of Kansas Medical Center, 3901 Rainbow Blvd, MS 2028, Kansas City, KS 66160. Tel: 913‐945‐8083 Fax: 913‐945‐6650 E‐mails: yoskaly@gmail.com; ylazofernandez@kumc.edu

The authors have no conflict of interests to disclose.

Subject:

Research Funding:

This study was funded by National Institute of Diabetes and Digestive and Kidney Diseases grants DK 104125 (to S.M.W), T32 DK‐07656 (to Y.L.F., B.M.W,. and A.Y.P), and minority supplement to DK 104125 (to Y.L.F.).

Keywords:

  • Blood collection
  • jugular catheter
  • mice
  • pendrin
  • survival surgery

Blood collection in unstressed, conscious, and freely moving mice through implantation of catheters in the jugular vein: a new simplified protocol

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Journal Title:

Physiological Reports

Volume:

Volume 6, Number 21

Publisher:

, Pages e13904-e13904

Type of Work:

Article | Final Publisher PDF

Abstract:

Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. The mouse has become the most common mammalian animal model used in biomedical research. However, laboratory techniques used previously in rats and other larger animals to sample blood had to be adapted in mice due to their lower mouse plasma volume. Sampling is further confounded by the variability in plasma hormone and metabolite concentrations that can occur from the stress or the anesthesia that accompanies the collection. In this article, we describe in detail a protocol we developed for blood sampling in conscious, unrestrained mice. Our protocol implements the use of chronic indwelling catheters in the right external jugular vein, allowing the mice to recover fully in their home cages, untethered until the time of blood sampling. This protocol employs catheters that remain patent for days and does not require the purchase of expensive equipment. We validated this protocol by measuring the time course of plasma norepinephrine (NE) concentration during and after the relief of acute immobilization stress in wild type (WT) and pendrin knockout (KO) mice and compared these results with our previously published values. We found that following relief from immobilization stress, it takes longer for plasma NE concentration to return to basal levels in the pendrin KO than in the wild type mice. These results highlight the potential utility of this protocol and the potential role of pendrin in the neuroendocrine response to acute stress.

Copyright information:

© 2018 The Authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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