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Author Notes:

E-mail: alicia.smith@emory.edu

Conceptualization: Anne L. Dunlop, Elizabeth J. Corwin, Karen N. Conneely, Alicia K. Smith.

Data curation: Anne L. Dunlop, Dawayland Cobb, Alicia K. Smith.

Formal analysis: Anna K. Knight, Varun Kilaru, Alicia K. Smith.

Funding acquisition: Elizabeth J. Corwin, Alicia K. Smith.

Investigation: Anne L. Dunlop.

Methodology: Anna K. Knight, Karen N. Conneely

Project administration: Elizabeth J. Corwin.

Supervision: Anne L. Dunlop, Karen N. Conneely, Alicia K. Smith.

Writing – original draft: Anna K. Knight.

Writing – review & editing: Anne L. Dunlop, Varun Kilaru, Dawayland Cobb, Elizabeth J. Corwin, Karen N. Conneely, Alicia K. Smith.

We are grateful to the participants enrolled in this cohort who provided samples and medical record data for this study. We are grateful to Misti B. Patel for providing insights into the clinical relevance of this work.

Subjects:

Research Funding:

Research reported in this publication was supported in part by the National Institute on Minority Health and Health Disparities (NIMHD, https://nimhd.nih.gov) of the National Institutes of Health, award number R01MD009064 to AKS and ALD, and the National Institute of Nursing Research (NINR, https://www.ninr.nih.gov) of the National Institutes of Health, under award number R01NR014800 to ALD and EJC.

The study was also supported by the Office of the Director (grant UG3OD023318 to Anne L Dunlop) and the National Institute of General Medical Sciences (grant T32 GM 8490-24 to Ms. Anna K Knight).

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • BLOOD MONONUCLEAR-CELLS
  • BACTERIAL VAGINOSIS
  • PRETERM BIRTH
  • MICROARRAY
  • ARTHRITIS

Characterization of gene expression changes over healthy term pregnancies

Journal Title:

PLoS ONE

Volume:

Volume 13, Number 10

Publisher:

, Pages e0204228-e0204228

Type of Work:

Article | Final Publisher PDF

Abstract:

During pregnancy, women experience numerous physiological changes but, to date, there is limited published data that characterize accompanying changes in gene expression over pregnancy. This study sought to characterize the complexity of the transcriptome over the course of pregnancy among women with healthy pregnancies. Subjects provided a venous blood sample during early (6-15 weeks) and late (22-33 weeks) pregnancy, which was used to isolate peripheral blood mononuclear cells prior to RNA extraction. Gene expression was examined for 63 women with uncomplicated, term deliveries. We evaluated the association between weeks gestation at sample collection and expression of each transcript. Of the 16,311 transcripts evaluated, 439 changed over pregnancy after a Bonferroni correction to account for multiple comparisons. Genes whose expression increased over pregnancy were associated with oxygen transport, the immune system, and host response to bacteria. Characterization of changes in the transcriptome over the course of healthy term pregnancies may enable the identification of genes whose expression predicts complications or adverse outcomes of pregnancy.

Copyright information:

© 2018 Knight et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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