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Author Notes:

Corresponding author at: J.S. Temenoff; W.H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA. Tel.: 1 404 332 5026; fax: 1 404 894 4243., johnna.temenoff@bme.gatech.edu.

We would like to acknowledge the Bellamkonda lab, especially Dr. Balakrishna Pai and Dr. Tarun Saxena, for their assistance with the extrusion device.

We also wish to acknowledge the core facilities at the Parker H. Petit Institute for Bioengineering and Bioscience at the Georgia Institute of Technology for the use of their shared equipment, services, and expertise, especially Andrew Shaw for his help with confocal microscopy.

This content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Subject:

Research Funding:

This study was supported with funding from the National Science Foundation (NSF) Graduate Research Fellowship (DGE-1148903) to TER, the Georgia Tech Petit Scholar Program to BDP, NSF (DMR 1207045), and the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number R01AR063692.

Keywords:

  • Science & Technology
  • Technology
  • Engineering, Biomedical
  • Materials Science, Biomaterials
  • Engineering
  • Materials Science
  • Core-shell microparticles
  • Heparin
  • Hydrolytically degradable
  • Protein delivery
  • Controlled release
  • BONE MORPHOGENETIC PROTEIN-2
  • FIBROBLAST-GROWTH-FACTOR
  • MESENCHYMAL STEM-CELLS
  • HEPARIN DESULFATION
  • DELIVERY-SYSTEM
  • HYDROGELS
  • MICROSPHERES
  • DESIGN
  • DIFFERENTIATION
  • FABRICATION

Core-shell microparticles for protein sequestration and controlled release of a protein-laden core

Tools:

Journal Title:

Acta Biomaterialia

Volume:

Volume 56

Publisher:

, Pages 91-101

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Development of multifunctional biomaterials that sequester, isolate, and redeliver cell-secreted proteins at a specific timepoint may be required to achieve the level of temporal control needed to more fully regulate tissue regeneration and repair. In response, we fabricated core-shell heparin-poly(ethylene-glycol) (PEG) microparticles (MPs) with a degradable PEG-based shell that can temporally control delivery of protein-laden heparin MPs. Core-shell MPs were fabricated via a re-emulsification technique and the number of heparin MPs per PEG-based shell could be tuned by varying the mass of heparin MPs in the precursor PEG phase. When heparin MPs were loaded with bone morphogenetic protein-2 (BMP-2) and then encapsulated into core-shell MPs, degradable core-shell MPs initiated similar C2C12 cell alkaline phosphatase (ALP) activity as the soluble control, while non-degradable core-shell MPs initiated a significantly lower response (85 + 19% vs. 9.0 + 4.8% of the soluble control, respectively). Similarly, when degradable core-shell MPs were formed and then loaded with BMP-2, they induced a ∼7-fold higher C2C12 ALP activity than the soluble control. As C2C12 ALP activity was enhanced by BMP-2, these studies indicated that degradable core-shell MPs were able to deliver a bioactive, BMP-2-laden heparin MP core. Overall, these dynamic core-shell MPs have the potential to sequester, isolate, and then redeliver proteins attached to a heparin core to initiate a cell response, which could be of great benefit to tissue regeneration applications requiring tight temporal control over protein presentation. Statement of Significance Tissue repair requires temporally controlled presentation of potent proteins. Recently, biomaterial-mediated binding (sequestration) of cell-secreted proteins has emerged as a strategy to harness the regenerative potential of naturally produced proteins, but this strategy currently only allows immediate amplification and re-delivery of these signals. The multifunctional, dynamic core-shell heparin-PEG microparticles presented here overcome this limitation by sequestering proteins through a PEG-based shell onto a protein-protective heparin core, temporarily isolating bound proteins from the cellular microenvironment, and re-delivering proteins only after degradation of the PEG-based shell. Thus, these core-shell microparticles have potential to be a novel tool to harness and isolate proteins produced in the cellular environment and then control when proteins are re-introduced for the most effective tissue regeneration and repair.

Copyright information:

© 2016 Acta Materialia Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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