Honokiol protects skin cells against inflammation, collagenolysis, apoptosis, and senescence caused by cigarette smoke damage

• Authors: Adilson Costa, Emory University; Gustavo Facchini, KOLderma Clinical Trials Institite; Ana Lúcia T. A. Pinheiro, KOLderma Clinical Trials Institite; Michelle Sabrina da Silva, KOLderma Clinical Trials Institite; Michael Yi Bonner, Emory University; Jack Arbiser, Emory University; Samara Eberlin, KOLderma Clinical Trials Institite
• Journal Title: International Journal of Dermatology
• Volume: Volume 56, Number 7
• Publisher: Wiley: 12 months | 2017-07-01, Pages 754-761
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1111/ijd.13569
• Subjects: Health Sciences, Medicine and Surgery; Health Sciences, General
• Keywords: Science & Technology; Life Sciences & Biomedicine; Dermatology; ARYL-HYDROCARBON RECEPTOR; NECROSIS-FACTOR-ALPHA; WEIGHT NATURAL-PRODUCT; FIBROBLASTS IN-VITRO; KAPPA-B ACTIVATION; OXIDATIVE STRESS; CYCLE ARREST; HUMAN KERATINOCYTES; SIGNALING PATHWAY; MAGNOLIA-OBOVATA

Abstract

Background: Pollution, especially cigarette smoke, is a major cause of skin damage. Objectives: To assess the effects of the small molecule polyphenol, honokiol, on reversing cigarette smoke-induced damage in vitro to relevant skin cells. Methods: Keratinocytes (HaCat) cultures were exposed to cigarette smoke and, after 48 hours, IL-1α and IL-8 were measured in cell supernatants. Moreover, TIMP-2 production, apoptosis rate, and senescence β-galactosidase expression were evaluated in primary human foreskin fibroblasts (HFF-1) cultures. Results: Honokiol at 10 μm reduced IL-1α production by 3.4 folds (P < 0.05) and at 10 and 20 μm reduced IL-8 by 23.9% and 53.1% (P < 0.001), respectively, in HaCat keratinocytes. In HFF-1, honokiol restored TIMP-2 production by 96.9% and 91.9% (P < 0.001), respectively, at 10 and 20 μm, as well as reduced apoptosis by 47.1% (P < 0.001) and 41.3% (P < 0.01), respectively. Finally, honokiol reduced senescence-associated β-galactosidase expression in HFF-1. Conclusion: Honokiol protects both HFF-1 and HaCat against cigarette smoke-induced inflammation, collagenolysis, apoptosis, and senescence.