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Author Notes:

To whom correspondence should be addressed: Dept. of Biochemistry, Emory University School of Medicine, 1510 Clifton Rd., Atlanta, GA 30322., Tel.: 404-727-3561; Fax: 404-727-3746; E-mail:rkahn@emory.edu

A. A. I. conducted most of the experiments, analyzed the results, and wrote the paper.

R. A. K. and T. C. coordinated the study and wrote the paper with A. A. I. A. B. W. and Z. L. performed HPLC analysis. N. T. S. and D. M. D. performed LC-MS/MS.

All authors reviewed the results and approved the final version of the manuscript.

We thank Dr. James Hurley (National Institutes of Health) for the gift of the pLEXm vectors.

The authors declare that they have no conflicts of interest with the contents of this article.

The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Subjects:

Research Funding:

This work was supported by National Institutes of Health Grants R01 GM110663 (to A. A. I., R. A. K., and T. C.) and R01 NS064934 (to A. B. W. and Z. L.), Proteomics Core of the Emory Neuroscience NINDS Core Facilities Grant P30NS055077 (to R. A. K., N. T. S., and D. M. D.), and in part by the Emory Integrated Proteomics Core (EIPC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemistry & Molecular Biology
  • ADP-RIBOSYLATION FACTOR
  • ARF FAMILY GTPASES
  • BINDING PROTEINS
  • JOUBERT-SYNDROME
  • ACTIVATING PROTEIN
  • FACTOR-I
  • ACID PHOSPHOLIPIDS
  • P-LOOP
  • RAS
  • HYDROLYSIS

Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF)

Tools:

Journal Title:

Journal of Biological Chemistry

Volume:

Volume 292, Number 26

Publisher:

, Pages 11091-11108

Type of Work:

Article | Final Publisher PDF

Abstract:

Primary cilia play central roles in signaling during metazoan development. Several key regulators of ciliogenesis and ciliary signaling are mutated in humans, resulting in a number of ciliopathies, including Joubert syndrome (JS). ARL13B is a ciliary GTPase with at least three missense mutations identified in JS patients. ARL13B is a member of the ADP ribosylation factor family of regulatory GTPases, but is atypical in having a non-homologous, C-terminal domain of ∼20 kDa and at least one key residue difference in the consensus GTP-binding motifs. For these reasons, and to establish a solid biochemical basis on which to begin to model its actions in cells and animals, we developed preparations of purified, recombinant, murine Arl13b protein. We report results from assays for solutionbased nucleotide binding, intrinsic and GTPase-activating protein-stimulated GTPase, and ARL3 guanine nucleotide exchange factor activities. Biochemical analyses of three human missense mutations found in JS and of two consensus GTPase motifs reinforce the atypical properties of this regulatory GTPase. We also discovered that murine Arl13b is a substrate for casein kinase 2, a contaminant in our preparation from human embryonic kidney cells. This activity, and the ability of casein kinase 2 to use GTP as a phosphate donor, may be a source of differences between our data and previously published results. These results provide a solid framework for further research into ARL13B on which to develop models for the actions of this clinically important cell regulator.

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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