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Author Notes:

* E-mail: dpjones@emory.edu

Conceived and designed the experiments: JR DJ.

Performed the experiments: JR YP FS.

Analyzed the data: JR YP SL.

Contributed reagents/materials/analysis tools: DJ SL FS.

Wrote the paper: JR.

The authors would like to thank Dr. Kichun Lee for his assistance regarding statistical analyses of the data and Dr. Quinlyn Soltow, Dr. Barbara Corkey and James Galligan for their critical reviews and constructive criticism of this manuscript.

The authors have declared that no competing interests exist.

Subjects:

Research Funding:

This work was supported by funding from the National Institute of Environmental Health Sciences at the National Institutes of Health (R01 ES009047 to D.P.J.; T32 ES012870 and F32 ES019821 to J.R.R.).

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Detailed Mitochondrial Phenotyping by High Resolution Metabolomics

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Journal Title:

PLoS ONE

Volume:

Volume 7, Number 3

Publisher:

Type of Work:

Article | Final Publisher PDF

Abstract:

Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used “top-down” metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate statistical tests provided global metabolic information about mitochondria and showed that liver mitochondria possess a significant phenotype based on gender and genotype. The data also show that mitochondria contain a large number of unidentified chemicals.

Copyright information:

© Roede et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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