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Author Notes:

Address correspondence to Philip N. Rather (prather@emory.edu).

We are grateful to Katy Clemmer for the isolation of PM2199 and Katy Clemmer and Elizabeth Ohneck for their comments on the manuscript.


Research Funding:

This work was funded by Merit Review and Research Career Scientist awards from the Department of Veterans Affairs.

Regulation of the Swarming Inhibitor disA in Proteus mirabilis


Journal Title:

Journal of Bacteriology


Volume 195, Number 14


, Pages 3237-3243

Type of Work:

Article | Final Publisher PDF


The disA gene encodes a putative amino acid decarboxylase that inhibits swarming in Proteus mirabilis. 5′ rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify the disA promoter. The use of a disA-lacZ fusion indicated that FlhD4C2, the class I flagellar master regulator, did not have a role in disA regulation. The putative product of DisA, phenethylamine, was able to inhibit disA expression, indicating that a negative regulatory feedback loop was present. Transposon mutagenesis was used to identify regulators of disA and revealed that umoB (igaA) was a negative regulator of disA. Our data demonstrate that the regulation of disA by UmoB is mediated through the Rcs phosphorelay.

Copyright information:

© 2013, American Society for Microbiology. All Rights Reserved.

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