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Author Notes:

Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322. Phone: (404) 727-0402. Fax: (404) 727-8999. E-mail: jrscott@emory.edu.

A. Camilli, Editor

We thank David Sue for help in construction of the Δstk allele and Gordon Churchward for critical advice and encouragement through these studies.

We are grateful to John W. Scott for the statistical analyses of the murine infection data and to Carlos Moreno for help with microarray analysis.

Subjects:

Research Funding:

This work was supported in part by NIH grant AI20723.

Serine/Threonine Protein Kinase Stk Is Required for Virulence, Stress Response, and Penicillin Tolerance in Streptococcus pyogenes▿

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Journal Title:

Infection and Immunity

Volume:

Volume 79, Number 10

Publisher:

, Pages 4201-4209

Type of Work:

Article | Final Publisher PDF

Abstract:

Genes encoding one or more Ser/Thr protein kinases have been identified recently in many bacteria, including one (stk) in the human pathogen Streptococcus pyogenes (group A streptococcus [GAS]). We report that in GAS, stk is required to produce disease in a murine myositis model of infection. Using microarray and quantitative reverse transcription-PCR (qRT-PCR) studies, we found that Stk activates genes for virulence factors, osmoregulation, metabolism of α-glucans, and fatty acid biosynthesis, as well as genes affecting cell wall synthesis. Confirming these transcription studies, we determined that the stk deletion mutant is more sensitive to osmotic stress and to penicillin than the wild type. We discuss several possible Stk phosphorylation targets that might explain Stk regulation of expression of specific operons and the possible role of Stk in resuscitation from quiescence.

Copyright information:

© 2011, American Society for Microbiology. All Rights Reserved.

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