Expression Vectors for Acinetobacter baylyi ADP1

• Authors: Charles Daniel Murin, Emory University; Kristy Segal, Emory University; Anton Bryksin, Emory University; Ichiro Matsumura, Emory University
• Journal Title: Applied and Environmental Microbiology
• Volume: Volume 78, Number 1
• Publisher: American Society for Microbiology | 2012-01, Pages 280-283
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1128/AEM.05597-11
• Subjects: Chemistry, Biochemistry

Abstract

Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.