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Author Notes:

Address correspondence to Ichiro Matsumura, imatsum@emory.edu.

Present address: Charles Daniel Murin, Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, California, USA; Kristy Segal, Odum School of Ecology, University of Georgia, Athens, Georgia, USA.

We thank Valérie de Crécy-Lagard (University of Florida) and Laura Cuff (University of Georgia) for their advice and insightful comments.

Subject:

Research Funding:

This work was supported by grants from the NIH (1 R01 GM074264; and 1 R01 GM086824).

Expression Vectors for Acinetobacter baylyi ADP1

Tools:

Journal Title:

Applied and Environmental Microbiology

Volume:

Volume 78, Number 1

Publisher:

, Pages 280-283

Type of Work:

Article | Final Publisher PDF

Abstract:

Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.

Copyright information:

© 2012, American Society for Microbiology. All Rights Reserved.

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