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Author Notes:

Corresponding Authors: Mailing Address: Gary W. Miller, Emory University, 1518 Clifton Road, Atlanta, GA 30322, United States; Phone: 404-712-8582; E-mail: gary.miller@emory.edu (G.W.M.)

Mailing Address: Mark R. Prausnitz, Georgia Institute of Technology, 311 Ferst Dr. NW, Atlanta, GA 30332-0100, United States; Phone: 404-894-5135; E-mail: prausnitz@gatech.edu (M.R.P.)

Author Contributions: M.M.N. and P.S. contributed equally to this work. M.R.P., G.W.M., P.S., and M.M.N. developed the concept and design of" "the study. M.R.P., G.W.M., and D.P.J. supervised all research." "P.S. collected biological samples from study participants. V.T. and D.I.W. conducted metabolomics assays. M.M.N. performed metabolomics data analysis. M.M.N., P.S., and D.I.W. interpreted results of data analysis. M.M.N. and P.S. wrote the manuscript. M.M.N., P.S., M.R.P., and G.W.M. revised the manuscript. All authors edited and approved the final manuscript."

We wish to thank Donna Bondy for administrative support. Dr. Amit Pandya helped develop protocols and provided training for the suction blister procedure.

The authors declare no competing financial interest.

Subjects:

Research Funding:

This work was supported by grants P30 ES019776, U2C ES026560, and T32 ES012870-13 from the National Institutes of Health.

Keywords:

  • Science & Technology
  • Physical Sciences
  • Chemistry, Analytical
  • Chemistry
  • DERMAL INTERSTITIAL FLUID
  • POINT-OF-CARE
  • PROTEOMIC CHARACTERIZATION
  • CARDIOVASCULAR-DISEASE
  • MASS-SPECTROMETRY
  • UROCANIC ACID
  • HUMAN-SKIN
  • BIOMARKERS
  • DISCOVERY
  • CANCER

Human Suction Blister Fluid Composition Determined Using High-Resolution Metabolomics

Tools:

Journal Title:

Analytical Chemistry

Volume:

Volume 90, Number 6

Publisher:

, Pages 3786-3792

Type of Work:

Article | Final Publisher PDF

Abstract:

Interstitial fluid (ISF) surrounds the cells and tissues of the body. Since ISF has molecular components similar to plasma, as well as compounds produced locally in tissues, it may be a valuable source of biomarkers for diagnostics and monitoring. However, there has not been a comprehensive study to determine the metabolite composition of ISF and to compare it to plasma. In this study, the metabolome of suction blister fluid (SBF), which largely consists of ISF, collected from 10 human volunteers was analyzed using untargeted high-resolution metabolomics (HRM). A wide range of metabolites were detected in SBF, including amino acids, lipids, nucleotides, and compounds of exogenous origin. Various systemic and skin-derived metabolite biomarkers were elevated or found uniquely in SBF, and many other metabolites of clinical and physiological significance were well correlated between SBF and plasma. In sum, using untargeted HRM profiling, this study shows that SBF can be a valuable source of information about metabolites relevant to human health.

Copyright information:

© 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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