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Author Notes:

Corresponding author. Mailing address: Research Service (VAMC), Room 5A181, 1670 Clairmont Road, Decatur, GA 30033. Phone: (404) 728-7688. Fax: (404) 329-2210. E-mail: wshafer@emory.edu

We thank Christal Hembree for help with the PFGE analyses, P. Johnson for help with figures, and Lane Pucko for help with manuscript preparation.


Research Funding:

This work was supported by NIH grant AI043316 and funds from a VA Merit Award from the Medical Research Service of the Department of Veterans Affairs, both to W.M.S.

W.M.S. is the recipient of a Senior Research Career Scientist Award from the Veterans Affairs Research Service.

S.S. is supported by the Centers for Disease Control and Prevention's Emerging Infections Program.

Altered Growth, Pigmentation, and Antimicrobial Susceptibility Properties of Staphylococcus aureus Due to Loss of the Major Cold Shock Gene cspB


Journal Title:

Antimicrobial Agents and Chemotherapy


Volume 54, Number 6


, Pages 2283-2290

Type of Work:

Article | Final Publisher PDF


An insertional mutation made in the major cold shock gene cspB in Staphylococcus aureus strain COL, a methicillin-resistant clinical isolate, yielded a mutant that displayed a reduced capacity to respond to cold shock and many phenotypic characteristics of S. aureus small-colony variants: a growth defect at 37°C, a reduction in pigmentation, and altered levels of susceptibility to many antimicrobials. In particular, a cspB null mutant displayed increased resistance to aminoglycosides, trimethoprim-sulfamethoxazole, and paraquat and increased susceptibility to daptomycin, teicoplanin, and methicillin. With the exception of the increased susceptibility to methicillin, which was due to a complete loss of the type I staphylococcal cassette chromosome mec element, these properties were restored to wild-type levels by complementation when cspB was expressed in trans. Taken together, our results link a stress response protein (CspB) of S. aureus to important phenotypic properties that include resistance to certain antimicrobials.

Copyright information:

© 2010, American Society for Microbiology

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