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Author Notes:

To whom correspondence should be addressed. E-mail: lfinzi@physics.emory.edu.

We are grateful to Sachin Goyal for help with figures.

D. A. E. Lewis and S. Adhya, manuscript in preparation. Liebesny, P., Goyal, S., Dunlap, D., Family, F., and Finzi, L. (2010) J. Phys. Condens. Matter, in press. L. Finzi and D. D. Dunlap, unpublished data.

Subjects:

Research Funding:

This work was supported, in whole or in part, by National Institutes of Health Grant RGM084070A (to L. F.).

This work was also supported by the Italian Funding of Basic Research (FIRB) (to L. F. and D. D. D.), the Human Frontier Science Program, and the Emory University Research Council.

Keywords:

  • Biophysics/Single Molecule
  • DNA
  • DNA/Operators
  • DNA-Protein Interaction
  • DNA/Transcription
  • GENE/Regulation
  • Nucleic Acid/Structure
  • Transcription/Regulation

Single-molecule Approaches to Probe the Structure, Kinetics, and Thermodynamics of Nucleoprotein Complexes That Regulate Transcription

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Journal Title:

Journal of Biological Chemistry

Volume:

Volume 285, Number 25

Publisher:

, Pages 18973-18978

Type of Work:

Article | Final Publisher PDF

Abstract:

Single-molecule experimentation has contributed significantly to our understanding of the mechanics of nucleoprotein complexes that regulate epigenetic switches. In this minireview, we will discuss the application of the tethered-particle motion technique, magnetic tweezers, and atomic force microscopy to (i) directly visualize and thermodynamically characterize DNA loops induced by the lac, gal, and λ repressors and (ii) understand the mechanistic role of DNA-supercoiling and DNA-bending cofactors in both prokaryotic and eukaryotic systems.

Copyright information:

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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