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Author Notes:

To whom correspondence should be addressed: Department of Pediatrics Infectious Diseases, Emory University, 2015 Uppergate Dr., Atlanta, GA 30322. Tel.: 404-727-4652; Fax: 404-727-9223; E-mail: gmeliki@emory.edu.

We thank Dr. Erdem Karatekin (Yale University) for expert advice on techniques for supported bilayer formation and Matthew Smith (Lehigh University) for helpful hints in using the Speckle TrackerJ.

We also thank José Rino (Instituto de Medicina Molecular) and Tanay Desai (Emory University) for critical reading of the manuscript.

Subjects:

Research Funding:

This work was supported, in whole or in part, by National Institutes of Health Grant R01 AI053668 (to G. B. M.).

This work was also supported by Fundação para a Ciência e Tecnologia, Ministério da Educação e Ciência (FCT-MEC, Portugal) Project Grant PTDC/QUI-BIQ/104787/2008 (to N. C. S.) and by Fellowship SFRH/BD/42205/2007 (to P. M. M.).

Keywords:

  • Fluorescence
  • Membrane Bilayer
  • Membrane Fusion
  • Microscopic Imaging
  • Virus Entry
  • Fusion Pore
  • Hemifusion
  • Single Virus Imaging

Anionic Lipids Are Required for Vesicular Stomatitis Virus G Protein-mediated Single Particle Fusion with Supported Lipid Bilayers

Tools:

Journal Title:

Journal of Biological Chemistry

Volume:

Volume 288, Number 18

Publisher:

, Pages 12416-12425

Type of Work:

Article | Final Publisher PDF

Abstract:

Background: Regulation of virus entry by host lipids is poorly understood. Results: Imaging of low pH-mediated fusion between single vesicular stomatitis pseudoviruses and lipid bilayers revealed a striking reliance on anionic lipids. Conclusion: The dependence of fusion on late endosome-resident anionic lipids suggests a new means for regulating the virus entry sites. Significance: Reliance on specific lipids for fusion may shed light on future antiviral strategies.

Copyright information:

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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