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Author Notes:

To whom correspondence may be addressed: Dept. of Biochemistry, Emory University School of Medicine, O. Wayne Rollins Research Center, 1510 Clifton Rd., Ste. 4001, Atlanta, GA 30322. Tel.: 404-727-5962; Fax: 404-727-2738; E-mail: rdcummi@emory.edu

To whom correspondence may be addressed: Dept. of Biochemistry, Emory University School of Medicine, O. Wayne Rollins Research Center, 1510 Clifton Rd., Suite 4001, Atlanta, GA 30322., Tel.: Phone: 404-727-6155; Fax: 404-727-2738; E-mail: dfsmith@emory.edu

We thank Shelly Gulati (University of Oklahoma Health Sciences Center) for help on influenza virus preparations and Dr. Jamie Heimburg-Molinaro (Emory University School of Medicine) for manuscript editing and review.

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Research Funding:

This work was supported, in whole or in part, by National Institutes of Health Grants GM62116 (to the Consortium for Functional Glycomics) and RO1 GM085448 (to D. F. S.).

This work was also supported by National Science Foundation Grant MCB 0718948 (to M. A.-M.) and United States Department of Health and Human Services Contract HHSN266200700006C (to the NIAID Centers of Excellence for Influenza Research and Surveillance).

Keywords:

  • Biomarkers
  • Glycobiology
  • Glycomics
  • Microarray
  • Virus
  • Biomarkers for Human Embryonic Stem Cells
  • Functional Glycomics
  • Glycan Microarray
  • Human Milk Glycans
  • Virus Interactions

Functional Glycomic Analysis of Human Milk Glycans Reveals the Presence of Virus Receptors and Embryonic Stem Cell Biomarkers

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Journal Title:

Journal of Biological Chemistry

Volume:

Volume 287, Number 53

Publisher:

, Pages 44784-44799

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background: Recognition of human milk glycans (HMGs) by lectins, antibodies, and pathogens is poorly understood. Results: Microarrays of isolated HMGs exhibited specific binding to proteins and pathogens. Conclusion: HMG microarray interrogation and novel metadata-assisted glycan sequencing provide a functional glycomics approach to discovering HMG function. Significance: HMGs represent a potential “liquid innate immune system” that is specifically recognized by antibodies and pathogens.

Copyright information:

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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