About this item:

501 Views | 222 Downloads

Author Notes:

To whom correspondence should be addressed: Dept. of Pathology, Whitehead Bldg. 105E, Emory University, 615 Michael St., Atlanta, GA 30322. Tel.: 404-727-5953; Fax: 404-727-8538; E-mail: pathgb@emory.edu.

We thank Bob Barstead for RB2, a random primed C. elegans cDNA library, Andy Fire for C. elegans expression vectors, and Kozo Kaibuchi for bacterial and yeast expression vectors.

Some strains used in this work were provided by the Caenorhabditis Genetics Center, which is supported by the National Center for Research Resources of the National Institutes of Health.

Subject:

Research Funding:

This work was supported in part by National Institutes of Health Grant AR052133.

This work was also supported by a Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad (2000) (to H. Q.), grants from the Canadian Institutes for Health Research and the National Science and Engineering Research Council of Canada (to D. G. M.), and American Heart Association Grant 11GRNT7820000 (to G. M. B.).

Keywords:

  • C. elegans
  • Integrin
  • Muscle
  • Protein-Protein Interactions
  • Signal Transduction
  • ILK
  • Integrin
  • Kindlin
  • Muscle

A Molecular Mechanism for the Requirement of PAT-4 (Integrin-linked Kinase (ILK)) for the Localization of UNC-112 (Kindlin) to Integrin Adhesion Sites

Tools:

Journal Title:

Journal of Biological Chemistry

Volume:

Volume 287, Number 34

Publisher:

, Pages 28537-28551

Type of Work:

Article | Final Publisher PDF

Abstract:

Background: PAT-4 (ILK) is required for localization of UNC-112 (kindlin) to integrin adhesion sites. Results: N- and C-terminal halves of UNC-112 interact, and mutations abolishing this interaction restore the ability of a mutant UNC-112 that cannot bind PAT-4 to localize. Conclusion: UNC-112 exists in two conformations, and binding to PAT-4 converts UNC-112 to an open state. Significance: This molecular mechanism may be conserved among kindlins.

Copyright information:

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

Export to EndNote