Mutant Huntingtin in Glial Cells Exacerbates Neurological Symptoms of Huntington Disease Mice

• Authors: Jennifer W. Bradford, Emory University; Ji-Yeon Shin, Emory University; Meredith Roberts, Emory University; Chuan-En Wang, Emory University; Guoqing Sheng, Emory University; Shihua Li, Emory University; Xiao-Jiang Li, Emory University
• Journal Title: Journal of Biological Chemistry
• Volume: Volume 285, Number 14
• Publisher: American Society for Biochemistry and Molecular Biology | 2010-04-02, Pages 10653-10661
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1074/jbc.M109.083287
• Subjects: Biology, Genetics; Chemistry, Biochemistry; Health Sciences, General
• Keywords: Aging; Diseases/Aging; Diseases/Genetic; Diseases/Neurodegeneration; Diseases/Neurological; Gene/Transcription

Abstract

Huntington disease (HD) is caused by an expansion of the polyglutamine (polyQ) repeat (>37Q) in huntingtin (htt), and age of onset is inversely correlated with the length of the polyQ repeat. Mutant htt with expanded polyQ is ubiquitously expressed in various types of cells, including glia, but causes selective neurodegeneration. Our recent study demonstrated that expression of the N-terminal mutant htt with a large polyQ repeat (160Q) in astrocytes is sufficient to induce neurological symptoms in mice (Bradford, J., Shin, J. Y., Roberts, M., Wang, C. E., Li, X.-J., and Li, S. H. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 22480–22485). Because glia-neuron interactions are critical for maintaining the normal function and survival of neurons in the brain and because mutant htt is more abundant in neurons than in glial cells, it is important to investigate whether glial htt can still contribute to HD pathology when mutant htt is abundantly expressed in neuronal cells. We generated transgenic mice that express mutant htt with 98Q in astrocytes. Unlike our recently generated htt-160Q transgenic mice, htt-98Q mice do not show obvious neurological phenotypes, suggesting that the length of the polyQ repeat determines the severity of glial dysfunction. However, htt-98Q mice show increased susceptibility to glutamate-induced seizure. Mice expressing mutant htt in astrocytes were mated with N171-82Q mice that express mutant htt primarily in neuronal cells. Double transgenic mice expressing mutant htt in both neuronal and glial cells display more severe neurological symptoms and earlier death than N171-82Q mice. These findings indicate a role of glial mutant htt in exacerbating HD neuropathology and underscore the importance of improving glial function in treating HD.