About this item:

96 Views | 113 Downloads

Author Notes:

Correspondence to: Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine, McGaw M-330, 240 E. Huron St., Chicago, IL 60611-3010, United States; d-kamp@northwestern.edu (D.W. Kamp)

Seok-Jo Kim and Paul Cheresh contributed equally to this manuscript.

Subjects:

Research Funding:

This work was supported by VA Merit 2I01BX000786-05A2 and NIH grant RO1 ES020357 to David W. Kamp, NIH/NHLBI training Grant 2T32HL076139-11A1 to Renea P. Jablonski, and VA Merit 1I01BX001910 to C. Michael Hart.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemistry & Molecular Biology
  • Endocrinology & Metabolism
  • INDUCED PULMONARY-FIBROSIS
  • FLOW-CYTOMETRIC ANALYSIS
  • REACTIVE OXYGEN
  • OXIDATIVE STRESS
  • SUPEROXIDE DISMUTASE
  • DEATH PATHWAY
  • FREE-RADICALS
  • LIFE-SPAN
  • ASBESTOS
  • BLEOMYCIN

Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage

Show all authors Show less authors

Tools:

Journal Title:

Free Radical Biology and Medicine

Volume:

Volume 101

Publisher:

, Pages 482-490

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Rationale Alveolar epithelial cell (AEC) injury and mitochondrial dysfunction are important in the development of lung fibrosis. Our group has shown that in the asbestos exposed lung, the generation of mitochondrial reactive oxygen species (ROS) in AEC mediate mitochondrial DNA (mtDNA) damage and apoptosis which are necessary for lung fibrosis. These data suggest that mitochondrial-targeted antioxidants should ameliorate asbestos-induced lung. Objective To determine whether transgenic mice that express mitochondrial-targeted catalase (MCAT) have reduced lung fibrosis following exposure to asbestos or bleomycin and, if so, whether this occurs in association with reduced AEC mtDNA damage and apoptosis. Methods Crocidolite asbestos (100 µg/50 µL), TiO 2 (negative control), bleomycin (0.025 units/50 µL), or PBS was instilled intratracheally in 8–10 week-old wild-type (WT - C57Bl/6 J) or MCAT mice. The lungs were harvested at 21 d. Lung fibrosis was quantified by collagen levels (Sircol) and lung fibrosis scores. AEC apoptosis was assessed by cleaved caspase-3 (CC-3)/Surfactant protein C (SFTPC) immunohistochemistry (IHC) and semi-quantitative analysis. AEC (primary AT2 cells from WT and MCAT mice and MLE-12 cells) mtDNA damage was assessed by a quantitative PCR-based assay, apoptosis was assessed by DNA fragmentation, and ROS production was assessed by a Mito-Sox assay. Results Compared to WT, crocidolite-exposed MCAT mice exhibit reduced pulmonary fibrosis as measured by lung collagen levels and lung fibrosis score. The protective effects in MCAT mice were accompanied by reduced AEC mtDNA damage and apoptosis. Similar findings were noted following bleomycin exposure. Euk-134, a mitochondrial SOD/catalase mimetic, attenuated MLE-12 cell DNA damage and apoptosis. Finally, compared to WT, asbestos-induced MCAT AT2 cell ROS production was reduced. Conclusions Our finding that MCAT mice have reduced pulmonary fibrosis, AEC mtDNA damage and apoptosis following exposure to asbestos or bleomycin suggests an important role for AEC mitochondrial H 2 O 2 -induced mtDNA damage in promoting lung fibrosis. We reason that strategies aimed at limiting AEC mtDNA damage arising from excess mitochondrial H 2 O 2 production may be a novel therapeutic target for mitigating pulmonary fibrosis.

Copyright information:

© 2016 Published by Elsevier Inc.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Creative Commons License

Export to EndNote