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Author Notes:

Address correspondence to Eric Rogier, Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, 1600 Clifton Road, Atlanta, GA 30329. E-mail: wwx6@cdc.gov

We would like to thank the students, parents, and teachers who allowed us to conduct this work, as well as the Government of Mali.

We would also like to thank the research team, including Abdoulaye Sow, Seydou Samaké, Salif Ismaïla Traoré, Fatoumata Habib Traoré, Karim Diamoutene, Yacouba Sogore, Alpha Oumar Haidara, as well as Niélé Hawa Diarra and Samba Diop from the University of Bamako.

We also thanks the UNICEF, WaterAid, CARE, Oxfam, and Save the Children teams for their support, specifically Jérémie Toubkiss, Yagouba Diallo, Seydou Niafo, Touréba Keïta, Assitan Sogoré, Salimata Togola, Fatoumata Haïdara, Mamadou Diallo, Zoumana Cissé, Ousmane Haïdara, and Thierno Bocoum.


Research Funding:

This work was supported by Dubai Cares Foundation.


  • Science & Technology
  • Life Sciences & Biomedicine
  • Public, Environmental & Occupational Health
  • Tropical Medicine
  • TERM

Evaluation of Immunoglobulin G Responses to Plasmodium falciparum and Plasmodium vivax in Malian School Children Using Multiplex Bead Assay


Journal Title:

American Journal of Tropical Medicine and Hygiene


Volume 96, Number 2


, Pages 312-318

Type of Work:

Article | Final Publisher PDF


Malaria serology through assaying for IgG against Plasmodium spp. antigens provides evidence into the infection history for an individual. The multiplex bead assay (MBA) allows for detection of IgG against multiple Plasmodium spp., and can be especially useful in many regions where Plasmodium falciparum is of primary clinical focus, but other species are co-endemic. Dried blood spots were collected from 805 Malian children attending 42 elementary schools in the regions of Mopti, Sikasso, Koulikoro, and Bamako capital district, and IgG assayed by MBA. As southern Mali is known to be holoendemic for P. falciparum, merozoite surface protein 1 19-kDa subunit (MSP-1 42 ) and apical membrane antigen 1 (AMA-1) antigens were included for serology against this parasite. Responses to these antigens both provided high estimates for lifetime exposure, with 730 (90%) children with IgG antibodies for MSP-1 42 , 737 (91%) for AMA-1, and 773 (96%) positive for either or both. Also included was the antigen Plasmodium vivax MSP-1 19 , against which 140 (17.4%) children were found to have antibodies. Increases in antibody titers with older age were clearly seen with the P. falciparum antigens, but not with the P. vivax antigen, likely indicating more of a sporadic, rather than sustained transmission for this species. The MBA provides effective opportunities to evaluate malaria transmission through serological analysis for multiple Plasmodium species.

Copyright information:

© 2017 by The American Society of Tropical Medicine and Hygiene.

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