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Author Notes:

Correspondence to Joshua J. Anzinger, Department of Microbiology, University of the West Indies, Mona, Kingston, Jamaica. E-mail: joshua.anzinger@uwimona.edu.jm

Author contributions: T.R.B., C.S.P., A.L.L., and J.J.A. designed the study. T

.R.B. carried out experiments.

T.R.B and J.J.A. wrote the manuscript.

A.L.L., D.B.H., R.C.K., S.M.C, and C.S.P. provided technical expertise and manuscript suggestions.

All authors read and approved the manuscript.

Data in this manuscript were collected by the Women's Interagency HIV Study (WIHS).

Subjects:

Research Funding:

The study was supported by the Office of the Principal of the University of the West Indies, Mona and NIH grants U01 AI68636 to the AIDS Clinical Trial Group (A.L.L.) and 1R01HL126543, 1R01HL095140, R21-HL-120394 (R.C.K.).

The WIHS is funded primarily by the National Institute of Allergy and Infectious Diseases (NIAID), with additional co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), the National Cancer Institute (NCI), the National Institute on Drug Abuse (NIDA), and the National Institute on Mental Health (NIMH).

Targeted supplemental funding for specific projects is also provided by the National Institute of Dental and Craniofacial Research (NIDCR), the National Institute on Alcohol Abuse and Alcoholism (NIAAA), the National Institute on Deafness and other Communication Disorders (NIDCD), and the NIH Office of Research on Women's Health.

WIHS data collection is also supported by UL1-TR000004 (UCSF CTSA) and UL1-TR000454 (Atlanta CTSA).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Immunology
  • Infectious Diseases
  • Virology
  • cardiovascular disease
  • GLUT1
  • HIV
  • monocyte
  • T cell
  • T-CELL-ACTIVATION
  • CAROTID-ARTERY-DISEASE
  • GLUCOSE-METABOLISM
  • IMMUNE-RESPONSE
  • ATHEROSCLEROSIS
  • INFLAMMATION
  • INDIVIDUALS
  • MARKERS
  • EVENTS
  • COAGULATION

Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease

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Journal Title:

AIDS

Volume:

Volume 31, Number 2

Publisher:

, Pages 199-205

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Objective: People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes upregulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated the expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIVinfected women with subclinical CVD. Methods: Participants with more than 75th percentile (n-15) and less than 25th percentile (n=15) age-Adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 cell count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Results: Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, P=0.024) (66.4% vs. 48.5%, P=0.031) and CD38 (339MFI vs. 211MFI, P=0.002) (10.5%vs. 3.8%, P=0.0002) compared withwomen with lowIMT. High and lowIMT participants showed no differences inGLUT1 or CD38 expression on classical monocytes, nonclassical monocytes, CD4 and CD8 T lymphocytes. Conclusion: GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.

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