About this item:

374 Views | 506 Downloads

Author Notes:

Corresponding author: Department of Cell Biology, Emory University School of Medicine, 615 Michael St., Rm. 443, Whitehead Medical Research Bldg., Atlanta, GA 30322. E-mail: djkatz@emory.edu

We thank the epigenetic community at Emory University for their feedback, T. Lee for help in editing this article, and A. Ferguson-Smith and M. Bartolomei for feedback on the article.

In addition, we thank M. Bartolomei for providing the H19 assay and D. Cutler for bioinformatics assistance.

Subjects:

Research Funding:

J.A.W. was supported by the Biochemistry, Cell and Molecular Biology Training Grant (5T32GM008367).

The work was supported by a grant to D.J.K. from the National Science Foundation (IOS1354998).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Genetics & Heredity
  • bisulfite analysis
  • DNA methylation
  • imprinting
  • mouse
  • single nucleotide polymorphism
  • PRADER-WILLI-SYNDROME
  • ENDOCRINE DISRUPTORS
  • GENE
  • GENOME
  • EXPRESSION
  • DBSNP
  • ESTABLISHMENT
  • DEMETHYLASE
  • PROTEINS
  • DATABASE

A Resource for the Allele-Specific Analysis of DNA Methylation at Multiple Genomically Imprinted Loci in Mice

Tools:

Journal Title:

G3

Volume:

Volume 8, Number 1

Publisher:

, Pages 91-103

Type of Work:

Article | Final Publisher PDF

Abstract:

Genomically imprinted loci are expressed mono-allelically, dependent upon the parent of origin. Their regulation not only illuminates how chromatin regulates gene expression but also how chromatin can be reprogrammed every generation. Because of their distinct parent-of-origin regulation, analysis of imprinted loci can be difficult. Single nucleotide polymorphisms (SNPs) are required to accurately assess these elements allele specifically. However, publicly available SNP databases lack robust verification, making analysis of imprinting difficult. In addition, the allele-specific imprinting assays that have been developed employ different mouse strains, making it difficult to systemically analyze these loci. Here, we have generated a resource that will allow the allele-specific analysis of many significant imprinted loci in a single hybrid strain of Mus musculus. This resource includes verification of SNPs present within 10 of the most widely used imprinting control regions and allele-specific DNA methylation assays for each gene in a C57BL/6J and CAST/EiJ hybrid strain background.

Copyright information:

© 2018 Wasson et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote