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Author Notes:

Correspondence to: My N. Helms at mhelms@emory.edu

Subjects:

Research Funding:

This work was supported by NIH K99 HL09222601 and the S&R Foundation Ryaji Uneo Award awarded to MNH.

Keywords:

  • Animals
  • Instillation, Drug
  • Intubation, Intratracheal
  • Lung
  • Mice
  • Trachea

Direct tracheal instillation of solutes into mouse lung.

Tools:

Journal Title:

Journal of Visualized Experiments

Volume:

Number 42

Publisher:

Type of Work:

Article | Final Publisher PDF

Abstract:

Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice. Using this approach, one can target delivery of test solutes or solids (such as lung therapeutics, surfactants, viruses, and small oligonucleotides) into the distal lung. Tracheal instillations may be the preferred methodology, over inhalation protocols that may primarily target the upper respiratory tract and possibly expose the investigator to potentially hazardous substances. Additionally, in using the tracheal instillation protocol, animals can fully recover from the non-invasive procedure. This allows for making subsequent physiological measurements on test animals, or reinstallation using the same animal. The amount of instillate introduced into the lung must be carefully determined and osmotically balanced to ensure animal recovery. Typically, 30-75 μL instillate volume can be introduced into mouse lung.

Copyright information:

© 2010, Journal of Visualized Experiments

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