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Correspondence: Eldon E. Geisert, egeiser@emory.edu
Author contributions: FS and EG conceived the study in coordination with AE.
JW performed all ONC procedures.
YL was responsible for intravitreal injections.
RK assisted with surgeries and animal breeding and isolated RNA for all samples.
OM and AE conducted SCN and DRG isolation.
FS performed and analyzed all ddPCR reactions and RACE assays, compiled figures and wrote the paper with input from EG and AE.
All authors read and approved the final manuscript.
We want to thank Oskar Laur for help with cloning and Xinping Huang for her assistance in virus packaging as well as April B. Still and Micah Chrenek for mouse colony management.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This study was supported by NEI grants R01EY017841 (EG), P30EY06360 (Emory Vision Core, P. Michael Iuvone), NINDS Grant NS057190 (AE), Unrestricted Funds from Research to Prevent Blindness and Department of Defense (DoD) Grant W81XWH12-1-0255 (EG).
FS is supported by the institutional training grant T32EY007092-30 (P. Michael Iuvone, PI).
The project was supported in part by the Viral Vector Core of the Emory Neuroscience NINDS Core Facilities grant, P30NS055077, and in part by the Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine as one of the Emory Integrated Core Facilities.
Additional support was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award Number UL1TR000454.
© 2017 Struebing, Wang, Li, King, Mistretta, English and Geisert.