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Correspondence: David K. Cole, coledk@cardiff.ac.uk; Andrew K. Sewell, sewellak@cardiff.ac.uk
David K. Cole, Anna Fuller, and Garry Dolton contributed equally to this work.
Author contributions: DC, AF, GD, EZ, ML, KM, JLB, FM, CH, AB, JSB, JM, AJAS, KB, BE, and PR performed and/or directed experiments, analysed data, and critiqued the manuscript.
DC and AKS conceived, funded, and directed the project and wrote the manuscript.
The authors would like to thank Diamond Light Source for beam time (proposals mx4532-1, mx6232-1, mx6232-9 and mx6232-10), and the staff of beamlines DLS I03, DLS I04-1 and DLS I24 for assistance with crystal testing and data collection.
We are extremely grateful to Andrew McMichael for providing advice and encouragement and to Anton van der Merwe for discussions about the biophysics and mechanism of SL9 TCR escape.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by the UK Biotechnology and Biological Sciences Research Council (Grant BB/H001085/1).
AKS is a Wellcome Trust Senior Investigator. FM was funded by a Tenovus PhD studentship.
DC is a Wellcome Trust Research Career Development Fellow (WT095767).
PR was supported by a RCUK Fellowship, and supported in part by grant GM067079 from the National Institute of General Medical Sciences, National Institutes of Health.
JM is an Australian National Health and Medical Research Council (NHMRC) Career Development Fellow (GNT1131732).
BDE was funded by NIH (R01 AI096879).
© 2017 Cole, Fuller, Dolton, Zervoudi, Legut, Miles, Blanchfield, Madura, Ho lland, Bulek, Bridgeman, Miles, Schauenburg, Beck, Evavold, Rizkallah and Sewell.