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Author Notes:

To whom correspondence should be addressed. Tel: +1 404 727 4546; Email: acorbe2@emory.edu. Correspondence may also be addressed to Grace K. Pavlath. Tel: +1 404 727 3590; Email: gpavlat@emory.edu.

Proteomic analysis was performed by the Emory University School of Medicine Integrated Proteomics Core.

We thank Dr Kishore Kumar Jella for help with image acquisition.

Conflict of interest statement. None declared.

The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health.

Subjects:

Research Funding:

National Institutes of Health [5RO1AR06198705 to A.H.C. and G.K.P., 5F32AR06827 to K.E.V.]; Muscular Dystrophy Association [255856 to A.B. and 422006 to A.H.C.].

Funding for open access charge: National Institutes of Health.

This study was supported in part by the Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemistry & Molecular Biology
  • OCULOPHARYNGEAL MUSCULAR-DYSTROPHY
  • LONG NONCODING RNA
  • AMYOTROPHIC-LATERAL-SCLEROSIS
  • LARGE-SCALE ANALYSIS
  • PRE-MESSENGER-RNA
  • POLY(A)-BINDING PROTEIN
  • SKELETAL-MUSCLE
  • PUR-ALPHA
  • HNRNP-C
  • GENE-EXPRESSION

Nuclear poly(A) binding protein 1 (PABPN1) and Matrin3 interact in muscle cells and regulate RNA processing

Tools:

Journal Title:

Nucleic Acids Research

Volume:

Volume 45, Number 18

Publisher:

, Pages 10706-10725

Type of Work:

Article | Final Publisher PDF

Abstract:

The polyadenylate binding protein 1 (PABPN1) is a ubiquitously expressed RNA binding protein vital for multiple steps in RNA metabolism. Although PABPN1 plays a critical role in the regulation of RNA processing, mutation of the gene encoding this ubiquitously expressed RNA binding protein causes a specific form of muscular dystrophy termed oculopharyngeal muscular dystrophy (OPMD). Despite the tissue-specific pathology that occurs in this disease, only recently have studies of PABPN1 begun to explore the role of this protein in skeletal muscle. We have used co-immunoprecipitation and mass spectrometry to identify proteins that interact with PABPN1 in mouse skeletal muscles. Among the interacting proteins we identified Matrin 3 (MATR3) as a novel protein interactor of PABPN1. The MATR3 gene is mutated in a form of distal myopathy and amyotrophic lateral sclerosis (ALS). We demonstrate, that like PABPN1, MATR3 is critical for myogenesis. Furthermore, MATR3 controls critical aspects of RNA processing including alternative polyadenylation and intron retention. We provide evidence that MATR3 also binds and regulates the levels of long non-coding RNA (lncRNA) Neat1 and together with PABPN1 is required for normal paraspeckle function. We demonstrate that PABPN1 and MATR3 are required for paraspeckles, as well as for adenosine to inosine (A to I) RNA editing of CtnRNA in muscle cells. We provide a functional link between PABPN1 and MATR3 through regulation of a common lncRNA target with downstream impact on paraspeckle morphology and function. We extend our analysis to a mouse model of OPMD and demonstrate altered paraspeckle morphology in the presence of endogenous levels of alanine-expanded PABPN1. In this study, we report protein-binding partners of PABPN1, which could provide insight into novel functions of PABPN1 in skeletal muscle and identify proteins that could be sequestered with alanine-expanded PABPN1 in the nuclear aggregates found in OPMD.

Copyright information:

© The Author(s) 2017.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/).

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