About this item:

306 Views | 323 Downloads

Author Notes:

E-mail: brian_ward@urmc.rochester.edu (BW); ykoyanag@virus.kyoto-u.ac.jp (YK); baek_kim@urmc.rochester.edu (BK)

Conceived and designed the experiments: B. Kim, B.M. Ward, Y. Koyanagi, D. Kim.

Performed the experiments: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward, J. Tate.

Analyzed the data: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward, J. Tate.

Contributed reagents/materials/analysis tools: J.A. Hollenbaugh, P. Gee, M.B. Daly, J. Baker, S.M. Amie, N. Kasai, Y. Kanemura, B.M. Ward.

Wrote the paper: J.A. Hollenbaugh, P. Gee, B.M. Ward, Y. Koyanagi, B. Kim.

We thank Dr. Bernard Roizman (University of Chicago) for HSV F strain.

The authors have declared that no competing interests exist.

Subjects:

Research Funding:

This work was supported by NIH AI077401 (B. Kim), AI049781 (B. Kim), a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology (24115008) (to Y. Koyanagi) and Grant-in-Aid for a fellowship from the Japan Society for the Promotion of Science (to P. Gee), NIH AI067391 (to B.M. Ward), AI099712 (B.M. Ward) and Cellular, Biochemical, and Molecular Sciences Training Grant GM068411 (S.M. Amie).

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Microbiology
  • Parasitology
  • Virology
  • HERPES-SIMPLEX-VIRUS
  • TYPE-1 REVERSE-TRANSCRIPTASE
  • CD4(+) T-CELLS
  • VACCINIA VIRUS
  • THYMIDINE KINASE
  • DENDRITIC CELLS
  • HIV-1 RESTRICTION
  • INFECTION
  • PROTEIN
  • REPLICATION

Host Factor SAMHD1 Restricts DNA Viruses in Non-Dividing Myeloid Cells

Show all authors Show less authors

Journal Title:

PLoS Pathogens

Volume:

Volume 9, Number 6

Publisher:

, Pages e1003481-e1003481

Type of Work:

Article | Final Publisher PDF

Abstract:

SAMHD1 is a newly identified anti-HIV host factor that has a dNTP triphosphohydrolase activity and depletes intracellular dNTP pools in non-dividing myeloid cells. Since DNA viruses utilize cellular dNTPs, we investigated whether SAMHD1 limits the replication of DNA viruses in non-dividing myeloid target cells. Indeed, two double stranded DNA viruses, vaccinia and herpes simplex virus type 1, are subject to SAMHD1 restriction in non-dividing target cells in a dNTP dependent manner. Using a thymidine kinase deficient strain of vaccinia virus, we demonstrate a greater restriction of viral replication in non-dividing cells expressing SAMHD1. Therefore, this study suggests that SAMHD1 is a potential innate anti-viral player that suppresses the replication of a wide range of DNA viruses, as well as retroviruses, which infect non-dividing myeloid cells.

Copyright information:

© 2013 Hollenbaugh et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote